HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

[6]-Gingerol induces caspase-dependent apoptosis and prevents PMA-induced proliferation in colon cancer cells by inhibiting MAPK/AP-1 signaling.

Abstract
We report mechanism-based evidence for the anticancer and chemopreventive efficacy of [6]-gingerol, the major active principle of the medicinal plant, Ginger (Zingiber officinale), in colon cancer cells. The compound was evaluated in two human colon cancer cell lines for its cytotoxic effect and the most sensitive cell line, SW-480, was selected for the mechanistic evaluation of its anticancer and chemopreventive efficacy. The non-toxic nature of [6]-gingerol was confirmed by viability assays on rapidly dividing normal mouse colon cells. [6]-gingerol inhibited cell proliferation and induced apoptosis as evidenced by externalization of phosphatidyl serine in SW-480, while the normal colon cells were unaffected. Sensitivity to [6]-gingerol in SW-480 cells was associated with activation of caspases 8, 9, 3 &7 and cleavage of PARP, which attests induction of apoptotic cell death. Mechanistically, [6]-gingerol down-regulated Phorbol Myristate Acetate (PMA) induced phosphorylation of ERK1/2 and JNK MAP kinases and activation of AP-1 transcription factor, but had only little effects on phosphorylation of p38 MAP kinase and activation of NF-kappa B. Additionally, it complemented the inhibitors of either ERK1/2 or JNK MAP kinase in bringing down the PMA-induced cell proliferation in SW-480 cells. We report the inhibition of ERK1/2/JNK/AP-1 pathway as a possible mechanism behind the anticancer as well as chemopreventive efficacy of [6]-gingerol against colon cancer.
AuthorsE K Radhakrishnan, Smitha V Bava, Sai Shyam Narayanan, Lekshmi R Nath, Arun Kumar T Thulasidasan, Eppurathu Vasudevan Soniya, Ruby John Anto
JournalPloS one (PLoS One) Vol. 9 Issue 8 Pg. e104401 ( 2014) ISSN: 1932-6203 [Electronic] United States
PMID25157570 (Publication Type: Journal Article)
Chemical References
  • Anticarcinogenic Agents
  • Antineoplastic Agents, Phytogenic
  • Carcinogens
  • Catechols
  • Fatty Alcohols
  • Transcription Factor AP-1
  • gingerol
  • Extracellular Signal-Regulated MAP Kinases
  • JNK Mitogen-Activated Protein Kinases
  • Caspases
  • Tetradecanoylphorbol Acetate
Topics
  • Anticarcinogenic Agents (chemistry, pharmacology)
  • Antineoplastic Agents, Phytogenic (chemistry, pharmacology)
  • Apoptosis (drug effects)
  • Carcinogens (toxicity)
  • Caspases (metabolism)
  • Catechols (chemistry, pharmacology)
  • Cell Line, Tumor
  • Colon (drug effects, metabolism)
  • Colonic Neoplasms (chemically induced, drug therapy, metabolism, prevention & control)
  • Extracellular Signal-Regulated MAP Kinases (metabolism)
  • Fatty Alcohols (chemistry, pharmacology)
  • Ginger (chemistry)
  • Humans
  • JNK Mitogen-Activated Protein Kinases (metabolism)
  • Signal Transduction (drug effects)
  • Tetradecanoylphorbol Acetate (toxicity)
  • Transcription Factor AP-1 (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: