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Kinetics and localization of wound-induced DNA biosynthesis in potato tuber.

Abstract
Tuber wounding induces a cascade of biological responses that are involved in processes required to heal and protect surviving plant tissues. Little is known about the coordination of these processes, including essential wound-induced DNA synthesis, yet they play critical roles in maintaining marketability of the harvested crop and tubers cut for seed. A sensitive "Click-iT EdU Assay" employing incorporation of the thymidine analog, 5-ethynyl-2'-deoxyuridine (EdU), in conjunction with 4',6-diamindino-2-phenylindole (DAPI) counter labeling, was employed to objectively identify and determine the time course and spatial distribution of tuber nuclei that were wound-induced to enter S-phase of the cell cycle. Both labeling procedures are rapid and sensitive in situ. Following wounding, EdU incorporation (indicating DNA synthesis) was not detectable until after 12h, rapidly reached a maximum at about 18h and then declined to near zero at 48h. About 28% of the nuclei were EdU labeled at 18h reflecting the proportion of cells in S-phase of the cell cycle. During the ∼30h in which induced cells were progressing through S-phase, de novo DNA synthesis extended 7-8 cell layers below the wound surface. Cessation of nuclear DNA synthesis occurred about 4 d prior to completion of wound closing layer formation. Initiation of wound periderm development followed at 7 d, i.e. about 5 d after cessation of nuclear DNA biosynthesis; at this time the phellogen developed and meristematic activity was detected via the production of new phellem cells. Collectively, these results provide new insight into the coordination of wound-induced nucleic acid synthesis with associated tuber wound-healing processes.
AuthorsEdward C Lulai, Jonathan D Neubauer, Jeffrey C Suttle
JournalJournal of plant physiology (J Plant Physiol) Vol. 171 Issue 17 Pg. 1571-5 (Nov 01 2014) ISSN: 1618-1328 [Electronic] Germany
PMID25151125 (Publication Type: Journal Article)
CopyrightPublished by Elsevier GmbH.
Chemical References
  • DNA, Plant
  • Indoles
  • Plant Proteins
  • DAPI
Topics
  • Cell Nucleus (genetics, metabolism)
  • DNA, Plant (genetics, metabolism)
  • Gene Expression Regulation, Plant
  • Indoles
  • Kinetics
  • Models, Biological
  • Organ Specificity
  • Plant Proteins (genetics, metabolism)
  • Plant Tubers (cytology, genetics, physiology)
  • S Phase
  • Solanum tuberosum (cytology, genetics, physiology)
  • Staining and Labeling
  • Time Factors
  • Wounds and Injuries

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