Impact of alanyl-glutamine dipeptide on proliferative and inflammatory changes in jejunal mucosa after acute mesenteric ischemia.

Abstract

PURPOSE:

The aim of our study was to determinate the impact of dipeptide (alanyl-glutamine) administration on inflammatory and proliferative changes in jejunal mucosa after acute mesenteric ischemia.

METHODS:

Male Wistar rats (n=30) were divided into three groups: ischemia/reperfusion (IR) group which undergoes 60min of mesenteric ischemia and 1 or 24h of reperfusion (IR1, IR24, n=12). Groups with dipeptide administration (D+IR1, D+IR24, Dipeptiven con inf., i.v., 0.75 g/kg) prior to IR injury were followed by 1 and 24h of reperfusion. At the end of reperfusion period jejunal bioptic samples were obtained for histological (H&E), histochemical (Alcian blue) and immunohistochemical (anti-PCNA, anti-MPO) evaluations.

RESULTS:

Our results pointed out a significant (p<0.001) increase of histopathological injury score in IR1 group compared to D+IR1 group. Immunohistochemical evaluation showed that MPO-positivity was significantly increased in IR groups after 1 (p<0.001) as well as 24h of reperfusion (p<0.01) compared to dipeptide pretreated groups. Proliferative/reparatory rate was assessed using anti-PCNA antibody and showed a significant increase (p<0.01) in PCNA cell positivity in lamina propria in dipeptide treated group compared to IR group.

CONCLUSION:

In conclusion we may suggest that administration of alanyl-glutamine dipeptide prior to IR injury may help to protect small intestine and its mucous membrane integrity against insult such as intestinal ischemic/reperfusion injury presents.

• Authors: Milan Maretta, Štefan Tóth, Zuzana Jonecová, Jarmila Veselá
• Journal: Journal of pediatric surgery (J Pediatr Surg) Vol. 49 Issue 9 Pg. 1385-9 (Sep 2014) ISSN: 1531-5037 [Electronic] United States
• PMID: 25148743 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• Dipeptides
• Proliferating Cell Nuclear Antigen
• Peroxidase
• alanylglutamine

Topics

• Animals
• Apoptosis
• Dipeptides (pharmacology)
• Immunohistochemistry
• Inflammation (pathology)
• Intestinal Mucosa (chemistry, drug effects, pathology)
• Jejunum (chemistry, drug effects, pathology)
• Male
• Mesenteric Ischemia (pathology)
• Peroxidase (analysis)
• Proliferating Cell Nuclear Antigen (analysis)
• Rats, Wistar
• Reperfusion Injury (pathology)