Inhibition of microbial adhesion to plastic surface and human buccal epithelial cells by Rhodomyrtus tomentosa leaf extract.

Abstract

OBJECTIVE:

The adherence of oral pathogenic microorganisms to host tissues is the initial step for successful process of oral diseases. This study aimed to determine the effect of the Rhodomyrtus tomentosa leaf extract and rhodomyrtone, an antibacterial compound from R. tomentosa leaf, on adhesion of some oral pathogens to polystyrene plastic surface and human buccal epithelial cells.

METHODS:

The minimum inhibitory concentration (MIC) was evaluated using broth microdilution method. The microbial adhesion to the plastic surface and buccal cells was determined using microtiter plate method and microscopy technique.

RESULTS:

The ethanol extract of leaf demonstrated antibacterial activity against oral microorganisms including Staphylococcus aureus ATCC 25923, Streptococcus mutans (clinical isolate), and Candida albicans ATCC 90028 with the MIC values of 31.25, 15.62, and 1000μg/ml, respectively. Rhodomyrtone displayed activity with the MIC values of 0.78 and 0.39μg/ml against S. aureus ATCC 25923 and S. mutans, respectively. The MIC value of the compound against C. albicans ATCC 90028 was more than 100μg/ml which was the highest test concentration. All pathogenic microorganisms treated with the extract and rhodomyrtone at their subinhibitory concentrations resulted in a decrease in their adherence ability to both plastic surface and buccal cells.

CONCLUSION:

It is suggested that R. tomentosa extract and rhodomyrtone may be useful in therapy or as prophylaxis in infections involving oral pathogens.

• Authors: Surasak Limsuwan, Sirilux Homlaead, Sirimaporn Watcharakul, Sasitorn Chusri, Kotchakorn Moosigapong, Jongkon Saising, Supayang Piyawan Voravuthikunchai
• Journal: Archives of oral biology (Arch Oral Biol) Vol. 59 Issue 12 Pg. 1256-65 (Dec 2014) ISSN: 1879-1506 [Electronic] England
• PMID: 25146902 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ltd. All rights reserved.

Chemical References

• Anti-Bacterial Agents
• Plant Extracts
• Xanthones
• rhodomyrtone

Topics

• Anti-Bacterial Agents (pharmacology)
• Bacterial Adhesion (drug effects)
• Candida albicans (drug effects)
• Humans
• In Vitro Techniques
• Microbial Sensitivity Tests
• Mouth Mucosa (cytology)
• Plant Extracts (pharmacology)
• Plant Leaves
• Staphylococcus aureus (drug effects)
• Streptococcus mutans (drug effects)
• Xanthones