6,6'-Bieckol inhibits adipocyte differentiation through downregulation of adipogenesis and lipogenesis in 3T3-L1 cells.

Abstract

BACKGROUND:

Brown algae have been used for their nutritional value as well as a source of bioactive compounds with antioxidant, anti-inflammatory, antimicrobial and anti-obesity effects. Obesity is an important condition implicated in various diseases, including diabetes, hypertension, dyslipidemia and coronary heart disease. However, anti-obesity effects of Eisenia bicyclis remain unknown.

RESULTS:

We investigated the anti-obesity effects of 6,6'-bieckol, 6,8'-bieckol, 8,8'-bieckol, dieckol and phlorofucofuroeckol A isolated from E. bicyclis. Anti-obesity activity was evaluated by examining the inhibition of differentiation of 3T3-L1 adipocytes and the expression of peroxisome proliferator-activated receptor γ (PPARγ), CCATT/enhancer-binding protein α (C/EBPα) and sterol regulatory element binding protein-1c (SREBP-1c) at the mRNA and protein level. Differentiated 3T3-L1 cells were treated with the purified phlorotannins at concentrations of 10, 25 and 50 µg mL(-1) for 8 days. The results indicated that the purified phlorotannins suppressed the differentiation of 3T3-L1 adipocytes in a dose-dependent manner, without toxic effects. Among the five compounds, 6,6'-bieckol markedly decreased lipid accumulation and expression levels of PPARγ, C/EBPα, SREBP-1c (mRNA and protein), and fatty acid synthase and acyl-coA carboxylase (mRNA).

CONCLUSION:

These findings suggest that E. bicyclis suppressed differentiation of 3T3-L1 adipocyte through downregulation of adipogenesis and lipogenesis.

• Authors: Tae-Hyung Kwon, Yong-Xiang Wu, Jong-Shik Kim, Jung-Hee Woo, Kyu Tae Park, O Jun Kwon, Hyun-Ju Seo, Taewan Kim, Nyun-Ho Park
• Journal: Journal of the science of food and agriculture (J Sci Food Agric) Vol. 95 Issue 9 Pg. 1830-7 (Jul 2015) ISSN: 1097-0010 [Electronic] England
• PMID: 25142414 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 Society of Chemical Industry.

Chemical References

• Anti-Obesity Agents
• Benzofurans
• Dioxins
• PPAR gamma
• dieckol
• phlorofucofuroeckol A
• 6,6'-bieckol
• Fatty Acid Synthase, Type I
• Carbon-Carbon Ligases
• acyl-CoA carboxylase

Topics

• 3T3-L1 Cells
• Adipogenesis (drug effects)
• Animals
• Anti-Obesity Agents (adverse effects, chemistry, isolation & purification, pharmacology)
• Benzofurans (adverse effects, chemistry, isolation & purification, pharmacology)
• Carbon-Carbon Ligases (antagonists & inhibitors, genetics, metabolism)
• Cell Survival (drug effects)
• Dioxins (adverse effects, chemistry, isolation & purification, pharmacology)
• Down-Regulation (drug effects)
• Fatty Acid Synthase, Type I (antagonists & inhibitors, genetics, metabolism)
• Lipid Metabolism (drug effects)
• Mice
• Molecular Structure
• PPAR gamma (antagonists & inhibitors, genetics, metabolism)
• Pacific Ocean
• Phaeophyta (chemistry)
• Republic of Korea
• Seaweed (chemistry)
• Stereoisomerism