Synthesis of composites SBA-15 mesoporous particles carrying oxytocin and evaluation of their properties, functions, and in vitro biological activities.

Abstract

Using the organic template method, we have synthesized mesoporous SBA-15 particles and characterized them by scanning electron microscopy and transmission electron microscopy. The bone metabolism regulating hormone oxytocin (OT) was selected as a model for preparation of drug/SBA-15 complexes. The process of drug loading was studied using X-ray diffraction and nitrogen absorption methods. Optimal drug loading parameters were experimentally investigated. The kinetics of drug release from the carrier was evaluated. Finally, the extractions of SBA-15 particles were tested for cytotoxicity, in vitro hemolysis, and the direct attachment toxicity. Our findings suggest that SBA-15 materials have good biocompatibility. Moreover, we demonstrated that OT/SBA-15 complex can stimulate alkaline phosphatase activity in osteoblast cells. The study provides fundamental information for further in vivo drug-carrier testing.

• Authors: Ming Qian, Min Liu, Mengna Duan, Zhe Wu, Yanmin Zhou
• Journal: Cell biochemistry and biophysics (Cell Biochem Biophys) Vol. 71 Issue 1 Pg. 127-34 (Jan 2015) ISSN: 1559-0283 [Electronic] United States
• PMID: 25138676 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Drug Carriers
• SBA-15
• Oxytocin
• Silicon Dioxide
• Alkaline Phosphatase
• Nitrogen

Topics

• Adsorption
• Alkaline Phosphatase (metabolism)
• Cell Adhesion (drug effects)
• Cell Line, Tumor
• Chemistry Techniques, Synthetic
• Drug Carriers (chemical synthesis, chemistry, pharmacology, toxicity)
• Drug Liberation
• Hemolysis (drug effects)
• Humans
• Nitrogen (chemistry)
• Osteoblasts (cytology, drug effects)
• Oxytocin (chemistry)
• Porosity
• Silicon Dioxide (chemical synthesis, chemistry, pharmacology, toxicity)