Lactobacillus casei reduces susceptibility to type 2 diabetes via microbiota-mediated body chloride ion influx.

Abstract

Gut microbiota mediated low-grade inflammation is involved in the onset of type 2 diabetes (T2DM). In this study, we used a high fat sucrose (HFS) diet-induced pre-insulin resistance and a low dose-STZ HFS rat models to study the effect and mechanism of Lactobacillus casei Zhang in protecting against T2DM onset. Hyperglycemia was favorably suppressed by L. casei Zhang treatment. Moreover, the hyperglycemia was connected with type 1 immune response, high plasma bile acids and urine chloride ion loss. This chloride ion loss was significantly prevented by L. casei via upregulating of chloride ion-dependent genes (ClC1-7, GlyRα1, SLC26A3, SLC26A6, GABAAα1, Bestrophin-3 and CFTR). A shift in the caecal microflora, particularly the reduction of bile acid 7α-dehydroxylating bacteria, and fecal bile acid profiles also occurred. These change coincided with organ chloride influx. Thus, we postulate that the prevention of T2DM onset by L. casei Zhang may be via a microbiota-based bile acid-chloride exchange mechanism.

• Authors: Yong Zhang, Xiao Guo, Jianlin Guo, Qiuwen He, He Li, Yuqin Song, Heping Zhang
• Journal: Scientific reports (Sci Rep) Vol. 4 Pg. 5654 (Jul 18 2014) ISSN: 2045-2322 [Electronic] England
• PMID: 25133590 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bile Acids and Salts
• Blood Glucose
• Chlorides
• Cytokines
• GATA3 Transcription Factor
• Ion Channels
• T-Box Domain Proteins
• T-box transcription factor TBX21

Topics

• Animals
• Bile Acids and Salts (analysis)
• Blood Glucose (analysis)
• Chlorides (metabolism)
• Cytokines (metabolism)
• Diabetes Mellitus, Type 2 (etiology, veterinary)
• Disease Susceptibility
• GATA3 Transcription Factor (genetics, metabolism)
• Insulin Resistance
• Intestines (microbiology)
• Ion Channels (metabolism)
• Lacticaseibacillus casei (physiology)
• Liver (metabolism)
• Male
• Microbiota
• Obesity (complications)
• Rats
• Rats, Sprague-Dawley
• T-Box Domain Proteins (genetics, metabolism)