Expression of placenta growth factor, soluble fms-like tyrosine kinase-1, metal-responsive transcription factor-1, heme oxygenase 1 and hypoxia inducible factor-1α mRNAs in pre-eclampsia placenta and the effect of pre-eclampsia sera on their expression of choriocarcinoma cells.

Abstract

AIM:

We studied the effect of pre-eclampsia sera on the expression of placenta growth factor (PlGF), soluble fms-like tyrosine kinase-1 (sFlt-1), metal-responsive transcription factor-1 (MTF-1), heme oxygenase 1 (HO-1) and hypoxia inducible factor-1α (HIF-1α) mRNAs in JEG-3 cells (trophoblast-derived cells) and placenta from pre-eclampsia patients to investigate pre-eclampsia pathophysiology.

MATERIAL AND METHODS:

Placenta and serum samples were taken from pre-eclampsia and normal pregnancy patients. JEG-3 cells were cultured with pre-eclampsia and normal pregnant sera in 24-well tissue culture plates. RNA was purified from placental trophoblast cells and JEG-3 cells 24 h after incubation. The expression of mRNA was measured using real-time polymerase chain reaction.

RESULTS:

The expression of sFlt-1 mRNA increased, and that of PlGF and HO-1 mRNA decreased in JEG-3 cells after incubation with pre-eclampsia sera. The expression of PlGF mRNA decreased, and that of sFlt-1mRNA increased in pre-eclampsia placenta. The expression of MTF-1 and HO-1 mRNA decreased. A correlation was found between PlGF mRNA expression and the expression of MTF-1 and HIF-1α mRNA. A correlation between sFlt-1 and HIF-1α mRNA expression was also found.

CONCLUSION:

Changes in PlGF mRNA expression in pre-eclampsia placenta may relate to serum factors and the expression of MTF-1 and HIF-α mRNA. Changes in sFlt-1mRNA expression may relate to serum factors and the expression of HIF-α mRNA. We suggest that serum factors play a role in PlGF and sFlt-1 expression in pre-eclampsia placenta.

• Authors: Aki Maebayashi Asanuma, Tatsuo Yamamoto, Hiromitu Azuma, Erina Kato, Noriko Yamamoto, Takayuki Murase, Fumihisa Chishima, Manami Suzuki
• Journal: The journal of obstetrics and gynaecology research (J Obstet Gynaecol Res) Vol. 40 Issue 10 Pg. 2095-103 (Oct 2014) ISSN: 1447-0756 [Electronic] Australia
• PMID: 25132343 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 The Authors. Journal of Obstetrics and Gynaecology Research © 2014 Japan Society of Obstetrics and Gynecology.

Chemical References

• DNA-Binding Proteins
• HIF1A protein, human
• Hypoxia-Inducible Factor 1, alpha Subunit
• PGF protein, human
• Pregnancy Proteins
• RNA, Messenger
• Recombinant Proteins
• Transcription Factors
• transcription factor MTF-1
• Placenta Growth Factor
• HMOX1 protein, human
• Heme Oxygenase-1
• Vascular Endothelial Growth Factor Receptor-1

Topics

• Adult
• Cell Line, Tumor
• DNA-Binding Proteins (genetics, metabolism)
• Female
• Gene Expression Regulation, Developmental
• Heme Oxygenase-1 (genetics, metabolism)
• Humans
• Hypoxia-Inducible Factor 1, alpha Subunit (genetics, metabolism)
• Placenta (enzymology, metabolism)
• Placenta Growth Factor
• Pre-Eclampsia (blood, enzymology, metabolism)
• Pregnancy
• Pregnancy Proteins (genetics, metabolism)
• RNA, Messenger (metabolism)
• Recombinant Proteins (chemistry, metabolism)
• Solubility
• Transcription Factors (genetics, metabolism)
• Vascular Endothelial Growth Factor Receptor-1 (chemistry, genetics, metabolism)