Abstract | BACKGROUND AND OBJECTIVES: MATERIALS AND METHODS: Seven patients with AIHA were included in this study. Serological testing was performed using standard techniques. Classification of autoantibodies was performed by preincubation of the eluates from patients' red blood cells (RBCs) with monospecific anti-human globulin sera (mAHG). RESULTS: Strong autoagglutination of patients' RBCs was observed in six of seven cases, with the identification of panagglutinating serum antibodies in three patients. Initially, cold agglutinins with high thermal amplitude were suggested in four patients, and IgM warm autoantibodies were suggested in the remaining three patients. However, inhibition of the eluates revealed autoantibodies of IgA class in two patients, of IgM class in three other cases and of IgG class in two patients. The results of DAT were confusing due to paradoxical effects of mAHG and/or strong autoagglutination. CONCLUSION:
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Authors | T Bartolmäs, B Mayer, S Yürek, R Genth, A Salama |
Journal | Vox sanguinis
(Vox Sang)
Vol. 108
Issue 1
Pg. 58-63
(Jan 2015)
ISSN: 1423-0410 [Electronic] England |
PMID | 25130988
(Publication Type: Journal Article)
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Copyright | © 2014 International Society of Blood Transfusion. |
Chemical References |
- Autoantibodies
- Cryoglobulins
- cold agglutinins
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Topics |
- Anemia, Hemolytic, Autoimmune
(blood, immunology)
- Autoantibodies
(blood, immunology)
- Coombs Test
(standards)
- Cryoglobulins
(immunology)
- Erythrocytes
(immunology)
- Female
- Humans
- Male
- Middle Aged
- Sensitivity and Specificity
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