Protective effects of myricitrin against osteoporosis via reducing reactive oxygen species and bone-resorbing cytokines.

Abstract

Oxidative stress is a crucial pathogenic factor in the development of osteoporosis. Myricitrin, isolated from Myrica cerifera, is a potent antioxidant. We hypothesized that myricitrin possessed protective effects against osteoporosis by partially reducing reactive oxygen species (ROS) and bone-resorbing cytokines in osteoblastic MC3T3-E1 cells and human bone marrow stromal cells (hBMSCs). We investigated myricitrin on osteogenic differentiation under oxidative stress. Hydrogen peroxide (H2O2) was used to establish an oxidative cell injury model. Our results revealed that myricitrin significantly improved some osteogenic markers in these cells. Myricitrin decreased lipid production and reduced peroxisome proliferator-activated receptor gamma-2 (PPARγ2) expression in hBMSCs. Moreover, myricitrin reduced the expression of receptor activator of nuclear factor kappa-B ligand (RANKL) and IL-6 and partially suppressed ROS production. In vivo, we established a murine ovariectomized (OVX) osteoporosis model. Our results demonstrated that myricitrin supplementation reduced serum malondialdehyde (MDA) activity and increased reduced glutathione (GSH) activity. Importantly, it ameliorated the micro-architecture of trabecular bones in the 4th lumbar vertebrae (L4) and distal femur. Taken together, these results indicated that the protective effects of myricitrin against osteoporosis are linked to a reduction in ROS and bone-resorbing cytokines, suggesting that myricitrin may be useful in bone metabolism diseases, particularly osteoporosis.

• Authors: Qiang Huang, Bo Gao, Long Wang, Ya-Qian Hu, Wei-Guang Lu, Liu Yang, Zhuo-Jing Luo, Jian Liu
• Journal: Toxicology and applied pharmacology (Toxicol Appl Pharmacol) Vol. 280 Issue 3 Pg. 550-60 (Nov 01 2014) ISSN: 1096-0333 [Electronic] United States
• PMID: 25130202 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• Flavonoids
• Interleukin-6
• PPAR gamma
• RANK Ligand
• Reactive Oxygen Species
• Tnfsf11 protein, mouse
• myricitrin

Topics

• Adult
• Aged
• Animals
• Bone Density (physiology)
• Bone and Bones (cytology, metabolism)
• Cell Differentiation (drug effects)
• Cell Line
• Female
• Flavonoids (pharmacology)
• Histocytochemistry
• Humans
• Interleukin-6 (analysis, metabolism)
• Male
• Mice
• Mice, Inbred BALB C
• Middle Aged
• Osteoporosis (prevention & control)
• Oxidative Stress (drug effects, physiology)
• PPAR gamma (analysis, metabolism)
• RANK Ligand (analysis, metabolism)
• Random Allocation
• Reactive Oxygen Species (analysis, metabolism)