Glioma-derived ADAM10 induces regulatory B cells to suppress CD8+ T cells.

Abstract

CD8+ T cells play an important role in the anti-tumor activities of the body. The dysfunction of CD8+ T cells in glioma is unclear. This study aims to elucidate the glioma cell-derived ADAM10 (A Disintegrin and metalloproteinase domain-containing protein 10) in the suppression of CD8+ effector T cells by the induction of regulatory B cells. In this study, glioma cells were isolated from surgically removed glioma tissue and stimulated by Phorbol myristate acetage (PMA) in the culture. The levels of ADAM10 in the culture were determined by enzyme-linked immunosorbent assay. Immune cells were assessed by flow cytometry. The results showed that the isolated glioma cells express ADAM10, which was markedly up regulated after stimulated with PMA. The glioma-derived ADAM10 induced activated B cells to differentiate into regulatory B cells, the later suppressed CD8+ T cell proliferation as well as the induced regulatory T cells, which also showed the immune suppressor effect on CD8+ effector T cell proliferation. In conclusion, glioma cells produce ADAM10 to induce Bregs; the latter suppresses CD8+ T cells and induces Tregs.

• Authors: Zhuo-peng Ye, Hai-yong He, Hui Wang, Wen-sheng Li, Lun Luo, Zhen-chao Huang, Ying Guo
• Journal: PloS one (PLoS One) Vol. 9 Issue 8 Pg. e105350 ( 2014) ISSN: 1932-6203 [Electronic] United States
• PMID: 25127032 (Publication Type: Journal Article, Retracted Publication)

Chemical References

• Membrane Proteins
• Amyloid Precursor Protein Secretases
• ADAM Proteins
• ADAM10 Protein
• ADAM10 protein, human

Topics

• ADAM Proteins (physiology)
• ADAM10 Protein
• Amyloid Precursor Protein Secretases (physiology)
• B-Lymphocytes (physiology)
• B-Lymphocytes, Regulatory (immunology)
• CD8-Positive T-Lymphocytes (immunology)
• Glioma (enzymology, immunology)
• Humans
• Immune Tolerance
• Lymphocyte Activation
• Membrane Proteins (physiology)
• T-Lymphocytes, Regulatory (immunology)
• Tumor Cells, Cultured