Fluorescence turn-on detection of Sn2+ in live eukaryotic and prokaryotic cells.

Abstract

Sn(2+) is usually added to toothpaste to prevent dental plaque and oral disease. However, studies of its physiological role and bacteriostatic mechanism are restricted by the lack of versatile Sn(2+) detection methods applicable to live cells, including Streptococcus mutans. Here we report two Sn(2+) fluorescent probes containing a rhodamine B derivative as a fluorophore, linked via the amide moiety to N,N-bis(2-hydroxyethyl)ethylenediamine (R1) and tert-butyl carbazate group (R2), respectively. These probes can selectively chelate Sn(2+) and show marked fluorescence enhancement due to the ring open reaction of rhodamine induced by Sn(2+) chelation. The probes have high sensitivity and selectivity for Sn(2+) in the presence of various relevant metal ions. Particularly, both R1 and R2 can target lysosomes, and R2 can probe Sn concentrations in lysosomes with rather acidic microenvironment. Furthermore, these two probes have low toxicity and can be used as imaging probes for monitoring Sn(2+) not only in live KB cells (eukaryotic) but also in Streptococcus mutans cells (prokaryotic), which is a useful tool to study the physiological function of Sn(2+) in biological systems.

• Authors: Haichuang Lan, Ying Wen, Yunming Shi, Keyin Liu, Yueyuan Mao, Tao Yi
• Journal: The Analyst (Analyst) Vol. 139 Issue 20 Pg. 5223-9 (Oct 21 2014) ISSN: 1364-5528 [Electronic] England
• PMID: 25126650 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Ions
• Rhodamines
• Tin
• rhodamine B

Topics

• Cell Line, Tumor
• Humans
• Hydrogen-Ion Concentration
• Ions (chemistry)
• Lysosomes (chemistry, metabolism)
• Microscopy, Fluorescence
• Rhodamines (chemical synthesis, chemistry)
• Streptococcus mutans (chemistry, metabolism)
• Tin (analysis)