Preventive effects of Escherichia coli strain Nissle 1917 with different courses and different doses on intestinal inflammation in murine model of colitis.

Abstract

OBJECTIVE:

To analyze the in vivo effect of Escherichia coli Nissle 1917 (EcN) with different courses and different doses to Sprague-Dawley rats with trinitrobenzene sulfonic acid (TNBS)-induced colitis.

METHODS:

The probiotic was orally administered with different courses of treatment (with or without pre-administration) and different doses (10(7)-10(9) CFU/day) to Sprague-Dawley rats with TNBS-induced colitis. Therapeutic effects, levels of cytokine in serum, mRNA and protein expression were analyzed.

RESULTS:

Oral EcN administration after TNBS-induced improved colitis dose dependently. In parallel, a reduction of disease activity index and colonic MPO activity together with a decreased level of TNF-α and a trend of increased IL-10 expression was detected. Pre-administration of 10(7)CFU/day EcN to TNBS-treated rats resulted in a significant protection against inflammatory response and colons isolated from these rats exhibited a more pronounced expression of ZO-1 than the other groups. In the group of pre-administration of 10(9)CFU/day, the condition was not improved but deteriorated.

CONCLUSIONS:

This study convincingly demonstrates that pre-administration of probiotic EcN with low dose is able to protect colitis of rats and mediate up-regulation of ZO-1 expression, but long-term of high-dose EcN may do harm to colitis.

• Authors: Sumei Sha, Bin Xu, Xiangyun Kong, Ni Wei, Jian Liu, Kaichun Wu
• Journal: Inflammation research : official journal of the European Histamine Research Society ... [et al.] (Inflamm Res) Vol. 63 Issue 10 Pg. 873-83 (Oct 2014) ISSN: 1420-908X [Electronic] Switzerland
• PMID: 25118782 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Tjp1 protein, rat
• Tumor Necrosis Factor-alpha
• Zonula Occludens-1 Protein
• Interleukin-10
• Trinitrobenzenesulfonic Acid
• Peroxidase

Topics

• Animals
• Anti-Inflammatory Agents (therapeutic use)
• Colitis (chemically induced, metabolism, pathology, therapy)
• Colon (metabolism, pathology)
• Disease Models, Animal
• Escherichia coli
• Female
• Ileum (pathology)
• Interleukin-10 (blood)
• Intestinal Mucosa (pathology)
• Peroxidase (metabolism)
• Probiotics (therapeutic use)
• Rats, Sprague-Dawley
• Trinitrobenzenesulfonic Acid
• Tumor Necrosis Factor-alpha (blood)
• Zonula Occludens-1 Protein (metabolism)