Noninvasive detection of sleep/wake changes and cataplexy-like behaviors in orexin/ataxin-3 transgenic narcoleptic mice across the disease onset.

Abstract

Sleep and behavioral monitoring of young mice is necessary for understating the progress of symptoms in congenital and acquired diseases associated with sleep and movement disorders. In the current study, we have developed a non-invasive sleep monitoring system that identifies wake and sleep patterns of newborn mice using a simple piezoelectric transducer (PZT). Using this system, we have succeeded in detecting age-dependent occurrences and changes in sleep fragmentation of orexin/ataxin-3 narcoleptic mice (a narcoleptic mouse model with postnatal hypocretin/orexin cell death) across the disease onset. We also detected REM sleep/cataplexy patterns (i.e., immobility with clear heartbeat [IMHB] signals due to the flaccid posture) by the PZT system, and found that sudden onset of REM sleep-like episodes specifically occur in narcoleptic, but not in wild type mice, suggesting that these episodes are likely cataplexy. In contrast, gradual onset of IMHB likely reflects occurrence of REM sleep. In summary, we have shown that the PZT system is useful as a non-invasive sleep and behavior monitoring system to analyze the developmental aspects of sleep and movement disorders in mice models.

• Authors: Masatoshi Sato, Yohei Sagawa, Nobuhide Hirai, Shinichi Sato, Masashi Okuro, Samika Kumar, Takashi Kanbayashi, Tetsuo Shimizu, Noriaki Sakai, Seiji Nishino
• Journal: Experimental neurology (Exp Neurol) Vol. 261 Pg. 744-51 (Nov 2014) ISSN: 1090-2430 [Electronic] United States
• PMID: 25118620 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• Intracellular Signaling Peptides and Proteins
• Neuropeptides
• Nuclear Proteins
• Orexins
• Transcription Factors
• Ataxin-3
• Atxn3 protein, mouse

Topics

• Animals
• Animals, Newborn
• Ataxin-3
• Cataplexy (diagnosis, etiology)
• Disease Models, Animal
• Electroencephalography
• Electromyography
• Intracellular Signaling Peptides and Proteins (genetics)
• Mice
• Mice, Inbred C57BL
• Mice, Transgenic
• Narcolepsy (complications, genetics)
• Neuropeptides (genetics)
• Nuclear Proteins (genetics)
• Orexins
• Sleep (physiology)
• Sleep Wake Disorders (diagnosis, etiology)
• Transcription Factors (genetics)
• Transducers
• Wakefulness (physiology)