In vitro anticancer activity of loquat tea by inducing apoptosis in human leukemia cells.

Abstract

Fresh loquat leaves have been used as folk health herb in Asian countries for long time, although the evidence supporting their functions is still minimal. This study aimed to clarify the chemopreventive effect of loquat tea extract (LTE) by investigating the inhibition on proliferation, and underlying mechanisms in human promyelocytic leukemia cells (HL-60). LTE inhibited proliferation of HL-60 in a dose-dependent manner. Molecular data showed that the isolated fraction of LTE induced apoptosis of HL-60 as characterized by DNA fragmentation; activation of caspase-3, -8, and -9; and inactivation of poly(ADP)ribose polymerase. Moreover, LTE fraction increased the ratio of pro-apoptotic Bcl-2-associated X protein (Bax)/anti-apoptotic myeloid cell leukemia 1 (Mcl-1) that caused mitochondrial membrane potential loss and cytochrome c released to cytosol. Thus, our data indicate that LTE might induce apoptosis in HL-60 cells through a mitochondrial dysfunction pathway. These findings enhance our understanding for chemopreventive function of loquat tea.

• Authors: Phyu Phyu Khine Zar, Satoshi Yano, Kozue Sakao, Fumio Hashimoto, Takayuki Nakano, Makoto Fujii, De-Xing Hou
• Journal: Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 78 Issue 10 Pg. 1731-7 ( 2014) ISSN: 1347-6947 [Electronic] England
• PMID: 25118018 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Biphenyl Compounds
• Free Radical Scavengers
• MCL1 protein, human
• Myeloid Cell Leukemia Sequence 1 Protein
• Picrates
• Plant Extracts
• bcl-2-Associated X Protein
• 1,1-diphenyl-2-picrylhydrazyl

Topics

• Antineoplastic Agents (chemistry, pharmacology)
• Apoptosis (drug effects)
• Beverages (analysis)
• Biphenyl Compounds (chemistry)
• Cell Proliferation (drug effects)
• Eriobotrya (chemistry)
• Free Radical Scavengers (chemistry, pharmacology)
• HL-60 Cells
• Humans
• Leukemia (pathology)
• Membrane Potential, Mitochondrial (drug effects)
• Myeloid Cell Leukemia Sequence 1 Protein (metabolism)
• Picrates (chemistry)
• Plant Extracts (chemistry, pharmacology)
• Up-Regulation (drug effects)
• bcl-2-Associated X Protein (metabolism)