Inhibition of N-acetylglucosaminyltransferase V enhances sensitivity of radiotherapy in human prostate cancer.

Abstract

The purpose of this study was to investigate the relationship between N-acetylglucosaminyltransferase V (GnT-V) and radiation sensitivity of prostate cancer (PCa) cells both in vitro and in vivo. Firstly, the GnT-V expression was studied in 84 cases of PCa tissues, in which higher level of GnT-V was detected more frequently in the advanced tumors. Secondly, the GnT-V stably suppressed cell lines PCa/1079 (Lncap/1079 and PC3/1079) were constructed from PCa cell lines (Lncap and PC3) in vitro. Attenuation of GnT-V inhibited cell proliferation, migration and increased apoptosis, which resulted in enhanced radiation sensitivity of PCa cells. The underlying mechanism may be relevant to the increasing ratio of Bax/Bcl-2, the blocking transcription of NF-κB and the reduction of cell cycle G2-M arrest. Finally, in in vivo study, compared with control groups, the irradiated PCa xenograft nude mice of PCa/1079 indicated to reduce tumor-growth rate and enhance survival time. Summary, our studies showed that inhibition of GnT-V probably improved PCa cells' radiation sensitivity.

• Authors: Huiyi Huang, Wenxia Chen, Qiulian Liu, Ting Wei, Weiliang Zhu, Hui Meng, Linlang Guo, Jian Zhang
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 451 Issue 3 Pg. 345-51 (Aug 29 2014) ISSN: 1090-2104 [Electronic] United States
• PMID: 25117443 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• N-Acetylglucosaminyltransferases
• alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase

Topics

• Animals
• Apoptosis (radiation effects)
• Cell Line, Tumor
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Down-Regulation
• Humans
• Male
• Mice
• Mice, Nude
• N-Acetylglucosaminyltransferases (antagonists & inhibitors, biosynthesis)
• Prostatic Neoplasms (radiotherapy)
• Radiation Tolerance (drug effects, genetics)