Abstract |
We investigated the protective effects against acute renal failure (ARF) of Anemarrhena asphodeloides (AA) and performed simultaneous analysis of three compounds, neomangiferin (1), mangiferin (2), and isomangiferin (3) in AA using a high-performance liquid chromatography-photodiode array. To measure the protective effect of ARF, the levels of reactive oxygen species (ROS) and glutathione depletion were determined using a kit. HPLC analysis was performed using a Gemini Cia column at 40 degrees C. The mobile phase used gradient elution with 1.0% (v/v) aqueous acetic acid (A) and 1.0% (v/v) acetic acid in acetonitrile (B). The flow rate was 1.0 mL/min. In our assay, AA extract inhibits cisplatin-induced production of intracellular ROS. Pre-incubation of AA extracts (10-200 microg/mL) markedly maintained cell viability compared with controls in the noncisplatin-treated cells. Calibration curves of all compounds showed good linearity (r2 > or = 0.9992). Recoveries of the three compounds were 98.9-103.4%. The relative standard deviations of intra- and inter-day precision were 0.07-1.73% and 0.12-1.49%, respectively. The amounts of the three components were 1.22-20.63 mg/g. The AA extract has potential as a therapeutic agent for treatment of ARF. In addition, the established method will help to improve quality control of AA.
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Authors | Chang-Seob Seo, Hyekyung Ha, Young-Jung Kim, Ju-Young Jungb |
Journal | Natural product communications
(Nat Prod Commun)
Vol. 9
Issue 6
Pg. 829-32
(Jun 2014)
ISSN: 1934-578X [Print] United States |
PMID | 25115091
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Plant Extracts
- Reactive Oxygen Species
- Glutathione
- Cisplatin
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Topics |
- Anemarrhena
(chemistry)
- Animals
- Cell Line
- Cell Survival
(drug effects)
- Chromatography, High Pressure Liquid
(methods)
- Cisplatin
(toxicity)
- Glutathione
(metabolism)
- Phytotherapy
- Plant Extracts
(chemistry, pharmacology)
- Plant Roots
(chemistry)
- Plants, Medicinal
- Reactive Oxygen Species
(metabolism)
- Renal Insufficiency
(prevention & control)
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