Immunohistochemical Study of Glypican-3 and HepPar-1 in Differentiating Hepatocellular Carcinoma from Metastatic Carcinomas in FNA of the Liver.

Abstract

Hepatocellular carcinoma (HCC) remains a common malignant cancer worldwide, it is considered the fifth most common malignant cancer. On the other hand, metastatic tumors are widespread in the liver , with metastatic adenocarcinoma (MA) constituting the greatest part, therefore differentiation of HCC from MA is a frequent problem facing the pathologist especially in liver fine-needle aspiration biopsies. Evaluating the diagnostic value of glypican-3 (GPC-3) and HepPar-1 immunostaining in differentiating hepatocellular carcinoma from metastatic tumors in liver cell block material. Fourty eight cell blocks prepared from FNA from the liver ( 30 cases HCC, 18 cases metastatic carcinoma in liver) stained by Glypican -3 and HepPar-1 immunohistochemical markers. Glypican-3 was immunoexpressed in 97% of cases of HCC while all cases of metastatic carcinoma were negative. HepPar-1 was expressed in 93% of cases of HCC and 11% of metastatic carcinoma of the liver. In this study the sensitivity of GPC3 in the diagnosis of HCC in cytological material was 96.7% and the specificity was 100% while the sensitivity and specificity of HepPar-1 was 93.3% and 88.9% respectively. Immunohistochemical staining for GPC-3 in cell block material of the liver is highly sensitive and specific and it is a valuable tool capable of differentiating HCC from most of metastatic tumors of the liver.

• Authors: Taiseer R Ibrahim, Samar M Abdel-Raouf
• Journal: Pathology oncology research : POR (Pathol Oncol Res) Vol. 21 Issue 2 Pg. 379-87 (Apr 2015) ISSN: 1532-2807 [Electronic] Switzerland
• PMID: 25108409 (Publication Type: Comparative Study, Evaluation Study, Journal Article)

Chemical References

• Antigens, Neoplasm
• Biomarkers, Tumor
• Glypicans
• HepPar-1 protein, human

Topics

• Antigens, Neoplasm (metabolism)
• Biomarkers, Tumor (metabolism)
• Biopsy, Fine-Needle
• Carcinoma, Hepatocellular (diagnosis, metabolism, secondary)
• Cell Differentiation
• Colorectal Neoplasms (pathology)
• Diagnosis, Differential
• Female
• Glypicans (metabolism)
• Humans
• Immunohistochemistry (methods)
• Liver (pathology)
• Liver Neoplasms (diagnosis, metabolism, secondary)
• Male
• Middle Aged
• Neoplasm Metastasis (diagnosis)
• Pancreatic Neoplasms (pathology)
• Retrospective Studies
• Sensitivity and Specificity
• Stomach Neoplasms (pathology)