In vitro evaluation of antiproliferative effect of ethyl gallate against human oral squamous carcinoma cell line KB.

Abstract

Although some polyphenols are known to possess anticancer activity against different cancer cell lines through induction of apoptosis, the mode of antiproliferative effect of ethyl gallate against human oral squamous carcinoma cell line KB was not studied until now. Therefore, the antiproliferative effect of ethyl gallate was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay in comparison with the reference drug paclitaxel. Generation of reactive oxygen species, mitochondrial membrane potential loss, DNA damage and apoptosis were determined using 2,7-diacetyldichlorofluorescein fluorescence, uptake of rhodamine-123 by mitochondria, comet assay and acridine orange/ethidium bromide dual-dye staining method. Both ethyl gallate and paclitaxel exhibited cytotoxicity in a dose-dependent manner. The 50% inhibitory concentration for ethyl gallate was 30 and 20 μg/mL for paclitaxel. A volume of 50 μg/mL of ethyl gallate was found to be significantly effective (P < 0.05) in controlling the cancer cell proliferation leading to acute apoptosis.

• Authors: Shalini Mohan, Kalaivani Thiagarajan, Rajasekaran Chandrasekaran
• Journal: Natural product research (Nat Prod Res) Vol. 29 Issue 4 Pg. 366-9 ( 2015) ISSN: 1478-6427 [Electronic] England
• PMID: 25104086 (Publication Type: Journal Article)

Chemical References

• Oxidants
• Reactive Oxygen Species
• ethyl gallate
• Gallic Acid
• Paclitaxel

Topics

• Apoptosis (drug effects)
• Carcinoma, Squamous Cell (pathology)
• Cell Proliferation (drug effects)
• DNA Damage (drug effects)
• Gallic Acid (analogs & derivatives, pharmacology)
• Humans
• KB Cells
• Membrane Potential, Mitochondrial (drug effects)
• Oxidants (pharmacology)
• Paclitaxel (pharmacology)
• Reactive Oxygen Species (metabolism)