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D-α-tocopherol polyethylene glycol succinate-based redox-sensitive paclitaxel prodrug for overcoming multidrug resistance in cancer cells.

Abstract
To overcome the multidrug resistance (MDR) of P-glycoprotein (P-gp) substrate anticancer drugs, such as paclitaxel (PTX), a novel dual-functional prodrug, D-α-tocopherol polyethylene glycol succinate (TPGS) based PTX prodrug (TPGS-S-S-PTX), was synthesized here to fulfill the synergistic effect of P-gp inhibiting and intracellular redox-sensitive release. The prodrug could self-assemble into stable micelles in physiological environment with a diameter of ∼140 nm, while it disassociated in reductive condition and released PTX and TPGS active derivatives rapidly. High cell cytotoxicity in PTX-resistant human ovarian cell line A2780/T was observed with enhanced PTX accumulation due to the P-gp inhibition by the TPGS moiety. The IC50 of TPGS-S-S-PTX was 55% and 91% more effective than that of Taxol (clinical formulation of PTX) and uncleavable TPGS-C-C-PTX prodrug, respectively. This was found to be related with the increased apoptosis/necrosis and cell arrest in G2/M phase. In vivo evaluation of the TPGS-S-S-PTX prodrug exhibited an extended half-life, increased AUC (area under the concentration-time curve), enhanced tumor distribution and significant tumor growth inhibition with reduced side effects as compared to Taxol and TPGS-C-C-PTX. This prodrug has great potential in improving efficiency in the treatment of MDR tumors.
AuthorsYuling Bao, Yuanyuan Guo, Xiangting Zhuang, Dan Li, Bolin Cheng, Songwei Tan, Zhiping Zhang
JournalMolecular pharmaceutics (Mol Pharm) Vol. 11 Issue 9 Pg. 3196-209 (Sep 02 2014) ISSN: 1543-8392 [Electronic] United States
PMID25102234 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Antineoplastic Agents
  • Drug Carriers
  • Micelles
  • Prodrugs
  • Vitamin E
  • Polyethylene Glycols
  • tocophersolan
  • Paclitaxel
Topics
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 (metabolism)
  • Animals
  • Antineoplastic Agents (chemistry, pharmacology)
  • Apoptosis (drug effects)
  • Cell Line, Tumor
  • Chemistry, Pharmaceutical (methods)
  • Drug Carriers (chemistry)
  • Drug Resistance, Multiple (drug effects)
  • Drug Resistance, Neoplasm (drug effects)
  • Female
  • G2 Phase Cell Cycle Checkpoints (drug effects)
  • Half-Life
  • Humans
  • Mice
  • Micelles
  • Ovarian Neoplasms (drug therapy, metabolism)
  • Oxidation-Reduction
  • Paclitaxel (chemistry, pharmacology)
  • Polyethylene Glycols (chemistry)
  • Prodrugs (chemistry, pharmacology)
  • Rats
  • Rats, Sprague-Dawley
  • Vitamin E (analogs & derivatives, chemistry)

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