Rice prolamin extract ameliorates acute murine colitis by inhibiting nuclear factor-kappa B and modulating intestinal apoptosis and cell proliferation.

Abstract

We investigated the impact of rice prolamin extract (RPE) on lipopolysaccharide (LPS)-induced nuclear factor (NF)-κB signalling in intestinal epithelial cells and macrophages, and determined the therapeutic efficacy of RPE in acute murine colitis. The effect of RPE on LPS-induced NF-κB signalling and proinflammatory gene expression was evaluated by reverse transcription-polymerase chain reaction (RT-PCR), Western blotting, immunofluorescence and electrophoretic mobility shift assay (EMSA). The in-vivo efficacy of RPE was assessed in mice with 3% dextran sulphate sodium (DSS)-induced colitis. Apoptotic and cellular proliferative activities were evaluated by immunostaining with cleaved caspase-3 and proliferating cell nuclear antigen (PCNA) antibodies. RPE inhibited LPS-induced expression of monocyte chemotactic protein (MCP)-1, interleukin (IL)-6 and tumour necrosis factor (TNF)-alpha and LPS-induced NF-κB signalling in intestinal epithelial cells and macrophages. RPE-fed, DSS-exposed mice showed less weight loss, longer colon length and lower histological score compared to control diet-fed, DSS-exposed mice. Immunostaining analysis revealed a significant decrease of cleaved caspase-3 positive cells in RPE-fed, DSS-exposed mice compared to DSS-exposed mice. Also, the number of PCNA-positive cells within intact colonic crypts decreased significantly in RPE-fed, DSS-exposed mice compared to control diet-fed, DSS-exposed mice. DSS-induced NF-κB signalling was inhibited by RPE. RPE ameliorates intestinal inflammation by inhibiting NF-κB activation and modulating intestinal apoptosis and cell proliferation in an acute murine colitis.

• Authors: C-Y Chung, Y-L Park, N Kim, H-H Oh, D-S Myung, J-S Kim, S-B Cho, W-S Lee, H-S Kim, B-W Ahn, Y-E Joo
• Journal: Clinical and experimental immunology (Clin Exp Immunol) Vol. 178 Issue 3 Pg. 537-47 (Dec 2014) ISSN: 1365-2249 [Electronic] England
• PMID: 25098704 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 British Society for Immunology.

Chemical References

• NF-kappa B
• Plant Extracts
• Prolamins

Topics

• Acute Disease
• Animals
• Apoptosis (drug effects)
• Cell Proliferation (drug effects)
• Colitis (drug therapy)
• Intestines (drug effects)
• Male
• Mice
• Mice, Inbred C57BL
• NF-kappa B (antagonists & inhibitors)
• Oryza (chemistry)
• Plant Extracts (pharmacology, therapeutic use)
• Prolamins (pharmacology)