Effects of Smilaxchinoside A and Smilaxchinoside C, two steroidal glycosides from Smilax riparia, on hyperuricemia in a mouse model.

Abstract

The roots and rhizomes of Smilax riparia, called 'Niu-Wei-Cai' in traditional Chinese medicine, are believed to be effective in treating the symptoms of gout. However, the active constituents and their uricosuric mechanisms are unknown. In this study, we isolated two steroidal glycosides, named smilaxchinoside A and smilaxchinoside C, from the total saponins obtained from the ethanol extract of the roots of S. riparia. We then examined if these two compounds were effective in reducing serum uric acid levels in a hyperuricemic mouse model induced by potassium oxonate. We observed that these two steroidal glycosides possess potent uricosuric activities, and the observed effects accompanied the reduction of renal mURAT1 and the inhibition of xanthine oxidase, which contribute to the enhancement of uric acid excretion and the reduction of hyperuricemia-induced renal dysfunction. Smilaxchinoside A and smilaxchinoside C may have a clinical utility in treating gout and other medical conditions caused by hyperuricemia.

• Authors: Xiao-Hui Wu, Chong-Zhi Wang, Jun Zhang, Shu-Qing Wang, Lide Han, Yan-Wen Zhang, Chun-Su Yuan
• Journal: Phytotherapy research : PTR (Phytother Res) Vol. 28 Issue 12 Pg. 1822-8 (Dec 2014) ISSN: 1099-1573 [Electronic] England
• PMID: 25098402 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 John Wiley & Sons, Ltd.

Chemical References

• Drugs, Chinese Herbal
• Glucose Transport Proteins, Facilitative
• Glycosides
• Organic Anion Transport Protein 1
• Organic Anion Transporters
• Plant Extracts
• Saponins
• Slc22a12 protein, mouse
• Slc22a6 protein, mouse
• Slc2a9 protein, mouse
• Steroids
• Uricosuric Agents
• smilaxchinoside A
• smilaxchinoside C
• Uric Acid
• potassium oxonate
• Oxonic Acid
• Xanthine Oxidase

Topics

• Animals
• Disease Models, Animal
• Drugs, Chinese Herbal (pharmacology)
• Glucose Transport Proteins, Facilitative (metabolism)
• Glycosides (isolation & purification, pharmacology)
• Hyperuricemia (drug therapy)
• Kidney (drug effects)
• Male
• Mice
• Organic Anion Transport Protein 1 (metabolism)
• Organic Anion Transporters (metabolism)
• Oxonic Acid
• Plant Extracts (pharmacology)
• Plant Roots (chemistry)
• Saponins (pharmacology)
• Smilax (chemistry)
• Steroids (isolation & purification, pharmacology)
• Uric Acid (blood)
• Uricosuric Agents (isolation & purification, pharmacology)
• Xanthine Oxidase (metabolism)