Abstract | OBJECTIVES: METHODS: Hamsters were intraperitoneally (ip) infected with L. infantum (10(7) stationary phase promastigotes). On day 45 post- infection animals were treated ip with AmB (1 or 5 mg/kg/day), allicin (5 mg/kg/day) or a combination of AmB (1 mg/kg/day) + allicin (5 mg/kg/day) for 5 days. Animals were clinically and biopathologically monitored and the antibody response ( IgG, IgG1, IgG2) was determined. Parasite burdens were estimated by limiting dilution and AmB biodistribution was determined by HPLC in plasma, kidney, spleen and liver. RESULTS: No clinical signs or liver and kidney alterations were observed. AmB (1 mg/kg/day) did not clear the Leishmania infection and no parasites were detected in two animals treated with 5 mg/kg/day allicin. Combination therapy (5 mg/kg allicin + 1 mg/kg AmB) reduced the L. infantum burden by >95%. Antileishmanial activity of the combination was comparable (P < 0.05) to the standard AmB treatment (5 mg/kg). CONCLUSIONS:
Allicin alone (5 mg/kg/day for 5 days) significantly reduced the Leishmania burden in spleen and liver of infected hamsters. Co-administration of allicin (5 mg/kg/day for 5 days) and AmB (1 mg/kg/day for 5 days) showed a partial additive effect on the reduction of leishmanial burden in both target organs.
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Authors | M Jesús Corral, Dolores R Serrano, Inmaculada Moreno, J J Torrado, Mercedes Domínguez, José M Alunda |
Journal | The Journal of antimicrobial chemotherapy
(J Antimicrob Chemother)
Vol. 69
Issue 12
Pg. 3268-74
(Dec 2014)
ISSN: 1460-2091 [Electronic] England |
PMID | 25096077
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © The Author 2014. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: [email protected]. |
Chemical References |
- Antiprotozoal Agents
- Disulfides
- Sulfinic Acids
- allicin
- Amphotericin B
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Topics |
- Amphotericin B
(pharmacokinetics, therapeutic use)
- Animal Structures
(chemistry)
- Animals
- Antiprotozoal Agents
(pharmacokinetics, therapeutic use)
- Chromatography, High Pressure Liquid
- Cricetinae
- Disease Models, Animal
- Disulfides
- Drug Therapy, Combination
(methods)
- Female
- Leishmania infantum
(drug effects, isolation & purification)
- Leishmaniasis, Visceral
(drug therapy)
- Parasite Load
- Plasma
(chemistry)
- Sulfinic Acids
(pharmacokinetics, therapeutic use)
- Treatment Outcome
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