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The upregulated expression of OX40/OX40L and their promotion of T cells proliferation in the murine model of asthma.

AbstractOBJECTIVE:
To investigate whether the expression of OX40/OX40 ligand (OX40L) was upregulated in a murine model of asthma and their significance in the pathogenesis of asthma.
METHODS:
After an ovalbumin-sensitized/challenged murine model of asthma was established, the expressions of OX40, OX40L in peripheral blood mononuclear cells (PBMCs) and bronchoalveolar lavage fluid (BALF) cell pellets were measured. Then T cell proliferation was analyzed by cell counting kit-8 (CCK8), and the protein levels of OX40 and OX40L in the lungs were determined by immunohistochemistry. The concentrations of IL-4 and IFN-γ in BALF and T cell culture supernatant were evaluated by ELISA.
RESULTS:
The percentages of CD4(+)OX40(+), CD19(+)OX40L(+), F4/80(+)OX40L(+) in PBMCs and BALF cell pellets were higher in asthma group than in control group (all P<0.01). The proliferation capacity of T cells in asthma group was higher than that in control group (P<0.05). In asthma group, stimulation of OX40 by anti-OX40 mAb obviously promoted T cell proliferation and secretion of IL-4 and IFN-γ. Immunohistochemistry assay showed that OX40 and OX40L protein levels were higher in asthma group than those in control group (all P<0.05).
CONCLUSIONS:
The expressions of OX40 and OX40L were upregulated in the murine asthmatic model. The upregulation of OX40/OX40L signals could induce the proliferation and cytokines secretion of T cells in asthmatic mice, indicating that OX40/OX40L signal was involved in the pathogenesis of asthma.
AuthorsWei Lei, Da-Xiong Zeng, Can-Hong Zhu, Gao-Qin Liu, Xiu-Qin Zhang, Chang-Guo Wang, Qin Wang, Jian-An Huang
JournalJournal of thoracic disease (J Thorac Dis) Vol. 6 Issue 7 Pg. 979-87 (Jul 2014) ISSN: 2072-1439 [Print] China
PMID25093096 (Publication Type: Journal Article)

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