Abstract |
Sesquiterpenoid tussilagone (TUS) has a variety of pharmacological activities, such as anti-oxidant, anti- cancer, and anti-inflammatory activities. In this study, we investigated the effects of TUS on dendritic cell (DC) functions and the underlying mechanisms. TUS inhibited lipopolysaccharide (LPS)-induced activation of DCs, as shown by decrease in surface molecule expression, cytokine production, cell migration, and allo-T cell activation. In addition, TUS inhibited LPS-induced activation of NF-κB, MAPKs, and IRF-3 signalings in DCs, although it did not directly affect kinase activities of IRAK1/4, TAK1, and IKK, which suggests that TUS might indirectly inhibit TLR signaling in DCs. As a critical mechanism, we showed that TUS activated heme oxygenase-1 (HO-1), which degrades heme to immunosuppressive products, such as carbon monoxide and bilirubin. HO-1 inhibitor reversed the inhibitory activity of TUS in DCs. In conclusion, this study suggests that TUS inhibits DC function through the induction of HO-1.
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Authors | Yunsoo Park, Hwa Sun Ryu, Hong Kyung Lee, Ji Sung Kim, Jieun Yun, Jong Soon Kang, Bang Yeon Hwang, Jin Tae Hong, Youngsoo Kim, Sang-Bae Han |
Journal | International immunopharmacology
(Int Immunopharmacol)
Vol. 22
Issue 2
Pg. 400-8
(Oct 2014)
ISSN: 1878-1705 [Electronic] Netherlands |
PMID | 25091622
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier B.V. All rights reserved. |
Chemical References |
- Cytokines
- Membrane Proteins
- Reactive Oxygen Species
- Sesquiterpenes
- Tlr4 protein, rat
- Toll-Like Receptor 4
- Nitric Oxide
- tussilagone
- Heme Oxygenase-1
- Hmox1 protein, mouse
- Protein Kinases
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Topics |
- Animals
- Cytokines
(genetics, metabolism)
- Dendritic Cells
(drug effects, immunology, metabolism)
- Female
- Heme Oxygenase-1
(biosynthesis)
- Lymphocyte Culture Test, Mixed
- Membrane Proteins
(biosynthesis)
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Nitric Oxide
(metabolism)
- Protein Kinases
(metabolism)
- Reactive Oxygen Species
(metabolism)
- Sesquiterpenes
(pharmacology)
- Spleen
(cytology)
- T-Lymphocytes
(drug effects, immunology)
- Toll-Like Receptor 4
(antagonists & inhibitors)
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