Antioxidant effect of salvianolic acid B on hippocampal CA1 neurons in mice with cerebral ischemia and reperfusion injury.

Abstract

OBJETIVE:

To investigate the neuroprotective effects and underlying mechanisms of salvianolic acid B (Sal B) extracted from Salvia miltiorrhiza on hippocampal CA1 neurons in mice with cerebral ischemia reperfusion injury.

METHODS:

Forty male National Institute of Health (NIH) mice were randomly divided into 4 groups with 10 animals each, including the sham group, the model group, the SalB group (SalB 22.5 mg/kg) and the nimodipine (Nim) group (Nim 1 mg/kg). A mouse model of cerebral ischemia and reperfusion injury was established by bilateral carotid artery occlusion for 30 min followed by 24-h reperfusion. The malondialdehyde (MDA) content, the nitric oxide synthase (NOS) activity, the superoxide dismutase (SOD) activity and total antioxidant capability (T-AOC) of the pallium were determined by biochemistry methods. The morphologic changes and Bcl-2 and Bax protein expression in hippocampal CA1 neurons were observed by using hematoxylineosin staining and immunohistochemistry staining, respectively.

RESULTS:

In the SalB group, the MDA content and the NOS activity of the pallium in cerebral ischemia-reperfusion mice significantly decreased and the SOD activity and the T-AOC significantly increased, as compared with the model group (P<0.05 or P<0.01). The SalB treatment also rescued neuronal loss (P<0.01) in the hippocampal CA1 region, strongly promoted Bcl-2 protein expression (P<0.01) and inhibited Bax protein expression (P<0.05).

CONCLUSIONS:

SalB increases the level of antioxidant substances and decreases free radicals production. Moreover, it also improves Bcl-2 expression and reduces Bax expression. SalB may exert the neuroprotective effect through mitochondria-dependent pathway on hippocampal CA1 neurons in mice with cerebral ischemia and reperfusion injury and suggested that SalB represents a promising candidate for the prevention and treatment of ischemic cerebrovascular disease.

• Authors: Yu-feng Jiang, Zhi-qin Liu, Wei Cui, Wen-tong Zhang, Jia-pei Gong, Xi-mei Wang, Ying Zhang, Mei-juan Yang
• Journal: Chinese journal of integrative medicine (Chin J Integr Med) Vol. 21 Issue 7 Pg. 516-22 (Jul 2015) ISSN: 1672-0415 [Print] China
• PMID: 25081897 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Benzofurans
• bcl-2-Associated X Protein
• Malondialdehyde
• salvianolic acid B
• Nitric Oxide Synthase
• Superoxide Dismutase

Topics

• Animals
• Antioxidants (pharmacology, therapeutic use)
• Benzofurans (chemistry, pharmacology, therapeutic use)
• Brain Ischemia (complications, drug therapy)
• CA1 Region, Hippocampal (pathology)
• Cell Count
• Immunohistochemistry
• Male
• Malondialdehyde (metabolism)
• Mice
• Neurons (drug effects, pathology)
• Nitric Oxide Synthase (metabolism)
• Reperfusion Injury (complications, drug therapy)
• Superoxide Dismutase (metabolism)
• bcl-2-Associated X Protein (metabolism)