Suppression of human breast cancer cell metastasis by coptisine in vitro.

Abstract

BACKGROUND:

Coptisine, an isoquinoline alkaloid extracted from Coptidis rhizoma, has many biological activities such as antidiabetic, antimicrobial and antiviral actions. However, whether coptisine exerts anti-cancer metastasis effects remains unknown.

MATERIALS AND METHODS:

Effects of coptisine on highly metastatic human breast cancer cell MDA-MB-231 proliferation were evaluated by trypan blue assay and on cell adhesion, migration and invasion by gelatin adhesion, wound-healing and matrigel invasion chamber assays, respectively. Expression of two matrix metalloproteinases (MMPs), MMP-9, MMP-2 and their specific inhibitors tissue inhibitor of metalloproteinase 1 (TIMP-1) and tissue inhibitor of metalloproteinase 2 (TIMP-2) were analyzed by RT-PCR.

RESULTS:

Coptisine obviously inhibited adhesion to an ECM-coated substrate, wound healing migration, and invasion through the matrigel in MDA-MB-231 breast cancer cells. RT-PCR revealed that coptisine reduced the expression of the ECM degradation-associated gene MMP-9 at the mRNA level, and the expression of TIMP-1 was up-regulated in MDA-MB-231 cells, while the expression of MMP-2 and its specific inhibitor TIMP-2 was not affected.

CONCLUSIONS:

Taken together, our data showed that coptisine suppressed adhesion, migration and invasion of MDA-MB-231 breast cancer cells in vitro, the down-regulation of MMP-9 in combination with the increase of TIMP-1 possibly contributing to the anti-metastatic function. Coptisine might be a potential drug candidate for breast cancer therapy.

• Authors: Jing Li, Dong-Min Qiu, Shao-Hua Chen, Su-Ping Cao, Xue-Lan Xia
• Journal: Asian Pacific journal of cancer prevention : APJCP (Asian Pac J Cancer Prev) Vol. 15 Issue 14 Pg. 5747-51 ( 2014) ISSN: 2476-762X [Electronic] Thailand
• PMID: 25081696 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Drug Combinations
• Laminin
• Proteoglycans
• RNA, Messenger
• TIMP1 protein, human
• TIMP2 protein, human
• Tissue Inhibitor of Metalloproteinase-1
• coptisine
• Berberine
• matrigel
• Tissue Inhibitor of Metalloproteinase-2
• Collagen
• MMP2 protein, human
• Matrix Metalloproteinase 2
• MMP9 protein, human
• Matrix Metalloproteinase 9

Topics

• Berberine (analogs & derivatives, pharmacology)
• Breast Neoplasms (drug therapy, pathology)
• Cell Adhesion (drug effects)
• Cell Line, Tumor
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Collagen
• Drug Combinations
• Female
• Humans
• Laminin
• Matrix Metalloproteinase 2 (biosynthesis)
• Matrix Metalloproteinase 9 (biosynthesis, genetics)
• Neoplasm Invasiveness (pathology, prevention & control)
• Neoplasm Metastasis (drug therapy, prevention & control)
• Proteoglycans
• RNA, Messenger (biosynthesis)
• Tissue Inhibitor of Metalloproteinase-1 (biosynthesis)
• Tissue Inhibitor of Metalloproteinase-2 (biosynthesis)
• Wound Healing (drug effects)