Abstract | AIM: METHODOLOGY: Periapical inflammatory lesions were obtained from extracted human teeth and teeth which underwent periapical surgery. After their histopathological categorization as periapical granulomas (n = 20), they were examined by immunohistochemistry using human LEPR monoclonal antibodies. LEPR mRNA expression was also determined by quantitative real-time PCR (qRT-PCR), and the amount of LEPR protein was analysed by immunoblot. RESULTS: All granuloma samples expressed LEPR. Amongst inflammatory cells, only macrophages showed expression of LEPR. Western blot analysis revealed the presence in the samples of a protein with apparent molecular weight of ~120 kDa, corresponding to the estimated molecular weight of LEPR. The qRT-PCR analysis demonstrated the expression of LEPR mRNA, corresponding the size of the amplified fragment (338 bp), assessed by agarose gel electrophoresis, to that of LEPR mRNA. CONCLUSIONS:
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Authors | J Martín-González, A Carmona-Fernández, A Pérez-Pérez, F Sánchez-Jiménez, V Sánchez-Margalet, J J Segura-Egea |
Journal | International endodontic journal
(Int Endod J)
Vol. 48
Issue 6
Pg. 611-8
(Jun 2015)
ISSN: 1365-2591 [Electronic] England |
PMID | 25081278
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 International Endodontic Journal. Published by John Wiley & Sons Ltd. |
Chemical References |
- LEPR protein, human
- RNA, Messenger
- Receptors, Leptin
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Topics |
- Aged
- Blotting, Western
- Electrophoresis, Agar Gel
- Female
- Humans
- Immunohistochemistry
- Male
- Middle Aged
- Periapical Granuloma
(metabolism, surgery)
- RNA, Messenger
(metabolism)
- Real-Time Polymerase Chain Reaction
- Receptors, Leptin
(metabolism)
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