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Expression and immunohistochemical localization of leptin receptor in human periapical granuloma.

AbstractAIM:
To investigate the expression and immunohistochemical localization of leptin receptor (LEPR) in human periapical granulomas.
METHODOLOGY:
Periapical inflammatory lesions were obtained from extracted human teeth and teeth which underwent periapical surgery. After their histopathological categorization as periapical granulomas (n = 20), they were examined by immunohistochemistry using human LEPR monoclonal antibodies. LEPR mRNA expression was also determined by quantitative real-time PCR (qRT-PCR), and the amount of LEPR protein was analysed by immunoblot.
RESULTS:
All granuloma samples expressed LEPR. Amongst inflammatory cells, only macrophages showed expression of LEPR. Western blot analysis revealed the presence in the samples of a protein with apparent molecular weight of ~120 kDa, corresponding to the estimated molecular weight of LEPR. The qRT-PCR analysis demonstrated the expression of LEPR mRNA, corresponding the size of the amplified fragment (338 bp), assessed by agarose gel electrophoresis, to that of LEPR mRNA.
CONCLUSIONS:
Human periapical granulomas express LEPR. In periapical granulomas, only macrophages showed expression of LEPR. This finding suggests that leptin can play a role in inflammatory and immune periapical responses.
AuthorsJ Martín-González, A Carmona-Fernández, A Pérez-Pérez, F Sánchez-Jiménez, V Sánchez-Margalet, J J Segura-Egea
JournalInternational endodontic journal (Int Endod J) Vol. 48 Issue 6 Pg. 611-8 (Jun 2015) ISSN: 1365-2591 [Electronic] England
PMID25081278 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2014 International Endodontic Journal. Published by John Wiley & Sons Ltd.
Chemical References
  • LEPR protein, human
  • RNA, Messenger
  • Receptors, Leptin
Topics
  • Aged
  • Blotting, Western
  • Electrophoresis, Agar Gel
  • Female
  • Humans
  • Immunohistochemistry
  • Male
  • Middle Aged
  • Periapical Granuloma (metabolism, surgery)
  • RNA, Messenger (metabolism)
  • Real-Time Polymerase Chain Reaction
  • Receptors, Leptin (metabolism)

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