Abstract | BACKGROUND: Adipocyte-secreted apelin contributes to decreased adiposity and to improved insulin resistance, but the mechanisms remain unknown. The present study aimed to assess if apelin-13 is an upstream signal regulation factor of aquaporin 7 (AQP7), a water- glycerol transporter present in the plasma membrane of adipocytes that plays a key role in the regulation of lipid accumulation. MATERIAL/METHODS: 3T3-L1 pre-adipocytes were induced to fully differentiated adipocytes; hypertrophic adipocytes were then induced using palmitate. The effects of apelin-13 on AQP7 expression in hypertrophic adipocytes were investigated before and after treatment with LY249002, a PI3K inhibitor. Accumulation of cytoplasmic triglycerides (TG) in hypertrophic adipocytes was also determined. RESULTS: We found that 0.1 mM of palmitate induced a model of hypertrophic adipocytes with a lower AQP7 expression (0.26±0.07 vs. 0.46±0.04, P<0.05). Apelin-13 100 nM or 1000 nM upregulated AQP7 mRNA expression (100 nM: 0.54±0.06 and 1000 nM: 0.58±0.09 vs. control: 0.33±0.04, both P<0.05), and decreased accumulation of cytoplasmic triglycerides in hypertrophic adipocytes. Pretreatment using 10 µM LY294002 prevented the increase in AQP7 expression observed when using apelin-13 alone ( apelin-13 + LY49002: 0.38±0.03 vs. apelin-13: 0.54±0.06, P<0.05), as well as the decreased cytoplasmic TG accumulation ( apelin-13 + LY294002: 3.79±0.04 µM per µg/ml vs. apelin-13: 3.32±0.08 µM per µg/ml, P<0.05). CONCLUSIONS:
Apelin-13 decreases lipid storage in hypertrophic adipocytes in vitro, possibly through the upregulation of AQP7 expression by the PI3K signaling pathway. Treatment using apelin-13 and AQP modulators might represent novel treatment strategies against obesity and its related complications.
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Authors | Mei Guo, Fu Chen, Tanfa Lin, Yanqiang Peng, Weiping Li, Xuxin Zhu, Ling Lin, Yongsong Chen |
Journal | Medical science monitor : international medical journal of experimental and clinical research
(Med Sci Monit)
Vol. 20
Pg. 1345-52
(Aug 01 2014)
ISSN: 1643-3750 [Electronic] United States |
PMID | 25080850
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adipokines
- Apelin
- Apln protein, mouse
- Aqp7 protein, mouse
- Aquaporins
- Azo Compounds
- Intercellular Signaling Peptides and Proteins
- Palmitates
- Phosphoinositide-3 Kinase Inhibitors
- oil red O
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Topics |
- 3T3-L1 Cells
- Adipocytes
(drug effects, metabolism)
- Adipokines
- Analysis of Variance
- Animals
- Apelin
- Aquaporins
(metabolism)
- Azo Compounds
- Gene Expression Regulation
(drug effects)
- Intercellular Signaling Peptides and Proteins
(metabolism, pharmacology)
- Lipid Metabolism
(drug effects)
- Mice
- Palmitates
- Phosphatidylinositol 3-Kinases
(metabolism)
- Phosphoinositide-3 Kinase Inhibitors
- Reverse Transcriptase Polymerase Chain Reaction
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