Abstract | BACKGROUND: METHODS: SCCKN were subcutaneously inoculated into the right flanks of NOD/SCID mice. Mice were treated with or without dietary glucosylceramides (300 mg/kg) daily for 14 consecutive days after confirmation of tumor progression. Microvessel areas around the tumor were assessed by hematoxylin- eosin staining and immunohistochemistry of CD31, and, as markers for angiogenesis, protein levels of VEGF, VEGF receptor-2, and HIF-1α were assessed by Western blotting. Mass spectrometry was performed to measure the levels of sphingolipids in mouse serum after treatment with dietary glucosylceramides. RESULTS: CONCLUSION:
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Authors | Hiroaki Yazama, Kazuyuki Kitatani, Kazunori Fujiwara, Misaki Kato, Mayumi Hashimoto-Nishimura, Katsuyuki Kawamoto, Kensaku Hasegawa, Hiroya Kitano, Alicja Bielawska, Jacek Bielawski, Toshiro Okazaki |
Journal | International journal of clinical oncology
(Int J Clin Oncol)
Vol. 20
Issue 3
Pg. 438-46
(Jun 2015)
ISSN: 1437-7772 [Electronic] Japan |
PMID | 25080062
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Angiogenesis Inhibitors
- Ceramides
- Glucosylceramides
- Hypoxia-Inducible Factor 1, alpha Subunit
- Vascular Endothelial Growth Factor A
- Vascular Endothelial Growth Factor Receptor-2
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Topics |
- Administration, Oral
- Angiogenesis Inhibitors
(administration & dosage)
- Animals
- Carcinoma, Squamous Cell
(diet therapy, metabolism)
- Ceramides
(biosynthesis)
- Glucosylceramides
(administration & dosage)
- Head and Neck Neoplasms
(diet therapy, metabolism)
- Humans
- Hypoxia-Inducible Factor 1, alpha Subunit
(biosynthesis)
- Mice
- Mice, Inbred NOD
- Mice, SCID
- Neovascularization, Pathologic
(diet therapy, metabolism)
- Vascular Endothelial Growth Factor A
(biosynthesis)
- Vascular Endothelial Growth Factor Receptor-2
(biosynthesis)
- Xenograft Model Antitumor Assays
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