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Altered expression of myelin-associated inhibitors and their receptors after traumatic brain injury in the mouse.

AbstractPURPOSE:
When central nervous system axons are injured, regeneration is partly inhibited by myelin-associated inhibitors (MAIs). Following traumatic brain injury (TBI) in the rat, pharmacological neutralisation of the MAIs Nogo-A and myelin-associated glycoprotein (MAG) resulted in improved functional outcome. In contrast, genetic or pharmacological neutralization of the MAI receptors Nogo-66 receptor 1 (NgR1) or paired-immunoglobulin like receptor-B (PirB) showed an unaltered or impaired outcome following TBI in mice. The aim of the present study was thus to evaluate the MAI expression levels following TBI in mice.
METHODS:
Quantitative reverse transcriptase PCR (qRT-PCR) was used to measure total RNA isolated from brains of young adult male C57BL/6 mice at one, three or seven days following controlled cortical impact TBI or sham injury. Hippocampal and neocortical tissue ipsi- and contralateral to the injury was analyzed for Nogo-A, oligodendrocyte-myelin glycoprotein (OMgp), MAG, and the MAI receptors PirB and NgR1, including its co-receptor Lingo1.
RESULTS:
Compared to sham-injured controls, PirB neocortical expression was significantly upregulated at one day and NgR1 expression downregulated at seven days post-TBI. In the hippocampus, transcriptional upregulation was observed in Nogo-A (one day post-injury), MAG and PirB at seven days post-injury. In contrast, the hippocampal transcripts of NgR1 and Lingo1 were decreased at seven days post-injury. The expression of OMgp was unaltered at all time points post-injury.
CONCLUSION:
These results suggest that early dynamic changes in MAI gene expression occur following TBI in the mouse, particularly in the hippocampus, which may play an inhibitory role for post-injury regeneration and plasticity.
AuthorsCharlotte Israelsson, Johanna Flygt, Elaine Åstrand, Olivia Kiwanuka, Henrik Bengtsson, Niklas Marklund
JournalRestorative neurology and neuroscience (Restor Neurol Neurosci) Vol. 32 Issue 5 Pg. 717-31 ( 2014) ISSN: 1878-3627 [Electronic] Netherlands
PMID25079982 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • GPI-Linked Proteins
  • Glial Fibrillary Acidic Protein
  • LINGO1 protein, mouse
  • Membrane Proteins
  • Myelin Proteins
  • Nerve Tissue Proteins
  • Nogo Proteins
  • Nogo Receptor 1
  • Oligodendrocyte-Myelin Glycoprotein
  • Pirb protein, mouse
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, Immunologic
  • Rtn4 protein, mouse
  • Rtn4 protein, rat
  • Rtn4r protein, mouse
  • glial fibrillary astrocytic protein, mouse
Topics
  • Animals
  • Brain (metabolism)
  • Brain Injuries (metabolism, pathology, physiopathology)
  • Disease Models, Animal
  • GPI-Linked Proteins (genetics, metabolism)
  • Gene Expression Regulation (physiology)
  • Glial Fibrillary Acidic Protein
  • Male
  • Membrane Proteins (genetics, metabolism)
  • Mice
  • Mice, Inbred C57BL
  • Myelin Proteins (genetics, metabolism)
  • Nerve Tissue Proteins (genetics, metabolism)
  • Nogo Proteins
  • Nogo Receptor 1
  • Oligodendrocyte-Myelin Glycoprotein (genetics, metabolism)
  • RNA, Messenger (metabolism)
  • Receptors, Cell Surface (genetics, metabolism)
  • Receptors, Immunologic (genetics, metabolism)
  • Time Factors

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