Abstract | PURPOSE: When central nervous system axons are injured, regeneration is partly inhibited by myelin-associated inhibitors (MAIs). Following traumatic brain injury (TBI) in the rat, pharmacological neutralisation of the MAIs Nogo-A and myelin-associated glycoprotein (MAG) resulted in improved functional outcome. In contrast, genetic or pharmacological neutralization of the MAI receptors Nogo-66 receptor 1 (NgR1) or paired- immunoglobulin like receptor-B (PirB) showed an unaltered or impaired outcome following TBI in mice. The aim of the present study was thus to evaluate the MAI expression levels following TBI in mice. METHODS: Quantitative reverse transcriptase PCR (qRT-PCR) was used to measure total RNA isolated from brains of young adult male C57BL/6 mice at one, three or seven days following controlled cortical impact TBI or sham injury. Hippocampal and neocortical tissue ipsi- and contralateral to the injury was analyzed for Nogo-A, oligodendrocyte-myelin glycoprotein (OMgp), MAG, and the MAI receptors PirB and NgR1, including its co-receptor Lingo1. RESULTS: Compared to sham-injured controls, PirB neocortical expression was significantly upregulated at one day and NgR1 expression downregulated at seven days post-TBI. In the hippocampus, transcriptional upregulation was observed in Nogo-A (one day post-injury), MAG and PirB at seven days post-injury. In contrast, the hippocampal transcripts of NgR1 and Lingo1 were decreased at seven days post-injury. The expression of OMgp was unaltered at all time points post-injury. CONCLUSION: These results suggest that early dynamic changes in MAI gene expression occur following TBI in the mouse, particularly in the hippocampus, which may play an inhibitory role for post-injury regeneration and plasticity.
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Authors | Charlotte Israelsson, Johanna Flygt, Elaine Åstrand, Olivia Kiwanuka, Henrik Bengtsson, Niklas Marklund |
Journal | Restorative neurology and neuroscience
(Restor Neurol Neurosci)
Vol. 32
Issue 5
Pg. 717-31
( 2014)
ISSN: 1878-3627 [Electronic] Netherlands |
PMID | 25079982
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- GPI-Linked Proteins
- Glial Fibrillary Acidic Protein
- LINGO1 protein, mouse
- Membrane Proteins
- Myelin Proteins
- Nerve Tissue Proteins
- Nogo Proteins
- Nogo Receptor 1
- Oligodendrocyte-Myelin Glycoprotein
- Pirb protein, mouse
- RNA, Messenger
- Receptors, Cell Surface
- Receptors, Immunologic
- Rtn4 protein, mouse
- Rtn4 protein, rat
- Rtn4r protein, mouse
- glial fibrillary astrocytic protein, mouse
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Topics |
- Animals
- Brain
(metabolism)
- Brain Injuries
(metabolism, pathology, physiopathology)
- Disease Models, Animal
- GPI-Linked Proteins
(genetics, metabolism)
- Gene Expression Regulation
(physiology)
- Glial Fibrillary Acidic Protein
- Male
- Membrane Proteins
(genetics, metabolism)
- Mice
- Mice, Inbred C57BL
- Myelin Proteins
(genetics, metabolism)
- Nerve Tissue Proteins
(genetics, metabolism)
- Nogo Proteins
- Nogo Receptor 1
- Oligodendrocyte-Myelin Glycoprotein
(genetics, metabolism)
- RNA, Messenger
(metabolism)
- Receptors, Cell Surface
(genetics, metabolism)
- Receptors, Immunologic
(genetics, metabolism)
- Time Factors
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