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Anthocyanins from fermented berry beverages inhibit inflammation-related adiposity response in vitro.

Abstract
Increased adiposity has been associated with macrophage infiltration into the adipose tissue which, in turn, leads to obesity comorbidities, including type 2 diabetes. The objective of this study was to evaluate the effect of anthocyanin (ANC)-enriched fractions from blackberry-blueberry beverages on inflammation and adipogenesis in an in vitro model of inflammation mimicking the pathologic interaction between adipocytes and macrophages. Blend ANCs inhibited secretion of nitric oxide (17.5%), tumor necrosis factor-alpha (TNF-α) (89.4%), and phosphorylated-p65 nuclear factor kappa-B (52.1%) in lipopolysaccharide (LPS)-induced RAW264.7 macrophages after 24 h. Blends reduced intracellular fat accumulation (28.2%) when applied during 3T3-L1 adipocyte differentiation and inhibited isoproterenol-induced lipolysis (18.6%) of mature 3T3-L1 cells. In addition, blend ANCs restored adiponectin-blunted gene expression induced by the TNF-α treatment (18.2%) and reduced the glycerol release (15.9%) induced by LPS-induced macrophage-conditioned media (CM) in adipocytes. Furthermore, blends slightly restored the insulin-induced glucose uptake of adipocytes, blunted by the CM treatment. In conclusion, ANCs from blueberry and blackberry dealcoholized fermented beverages are potential inhibitors of inflammation-related adiposity response and sensitizers of insulin signaling in adipocytes.
AuthorsDiego F Garcia-Diaz, Michelle H Johnson, Elvira G de Mejia
JournalJournal of medicinal food (J Med Food) Vol. 18 Issue 4 Pg. 489-96 (Apr 2015) ISSN: 1557-7600 [Electronic] United States
PMID25079118 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
Chemical References
  • Anthocyanins
  • Tumor Necrosis Factor-alpha
  • Nitric Oxide
Topics
  • 3T3-L1 Cells
  • Adipocytes (cytology, drug effects, immunology)
  • Adipogenesis (drug effects)
  • Adiposity (drug effects)
  • Animals
  • Anthocyanins (metabolism, pharmacology)
  • Beverages (analysis, microbiology)
  • Blueberry Plants (chemistry, metabolism, microbiology)
  • Fermentation
  • Fruit (chemistry, metabolism, microbiology)
  • Lipolysis
  • Macrophages (drug effects, immunology)
  • Mice
  • Nitric Oxide (immunology)
  • RAW 264.7 Cells
  • Rubus (chemistry, metabolism, microbiology)
  • Saccharomyces (metabolism)
  • Tumor Necrosis Factor-alpha (genetics, immunology)

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