Abstract |
Increased adiposity has been associated with macrophage infiltration into the adipose tissue which, in turn, leads to obesity comorbidities, including type 2 diabetes. The objective of this study was to evaluate the effect of anthocyanin (ANC)-enriched fractions from blackberry-blueberry beverages on inflammation and adipogenesis in an in vitro model of inflammation mimicking the pathologic interaction between adipocytes and macrophages. Blend ANCs inhibited secretion of nitric oxide (17.5%), tumor necrosis factor-alpha (TNF-α) (89.4%), and phosphorylated-p65 nuclear factor kappa-B (52.1%) in lipopolysaccharide (LPS)-induced RAW264.7 macrophages after 24 h. Blends reduced intracellular fat accumulation (28.2%) when applied during 3T3-L1 adipocyte differentiation and inhibited isoproterenol-induced lipolysis (18.6%) of mature 3T3-L1 cells. In addition, blend ANCs restored adiponectin-blunted gene expression induced by the TNF-α treatment (18.2%) and reduced the glycerol release (15.9%) induced by LPS-induced macrophage- conditioned media (CM) in adipocytes. Furthermore, blends slightly restored the insulin-induced glucose uptake of adipocytes, blunted by the CM treatment. In conclusion, ANCs from blueberry and blackberry dealcoholized fermented beverages are potential inhibitors of inflammation-related adiposity response and sensitizers of insulin signaling in adipocytes.
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Authors | Diego F Garcia-Diaz, Michelle H Johnson, Elvira G de Mejia |
Journal | Journal of medicinal food
(J Med Food)
Vol. 18
Issue 4
Pg. 489-96
(Apr 2015)
ISSN: 1557-7600 [Electronic] United States |
PMID | 25079118
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Anthocyanins
- Tumor Necrosis Factor-alpha
- Nitric Oxide
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Topics |
- 3T3-L1 Cells
- Adipocytes
(cytology, drug effects, immunology)
- Adipogenesis
(drug effects)
- Adiposity
(drug effects)
- Animals
- Anthocyanins
(metabolism, pharmacology)
- Beverages
(analysis, microbiology)
- Blueberry Plants
(chemistry, metabolism, microbiology)
- Fermentation
- Fruit
(chemistry, metabolism, microbiology)
- Lipolysis
- Macrophages
(drug effects, immunology)
- Mice
- Nitric Oxide
(immunology)
- RAW 264.7 Cells
- Rubus
(chemistry, metabolism, microbiology)
- Saccharomyces
(metabolism)
- Tumor Necrosis Factor-alpha
(genetics, immunology)
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