Evaluation of the sonosensitizing activities of 5-aminolevulinic acid and Sn(IV) chlorin e6 in tumor-bearing chick embryos.

Abstract

BACKGROUND:

Recently, 5-aminolevulinic acid (5-ALA), precursors of protoporphyrin IX (PpIX), and Sn(IV) chlorin e6 (SnCe6) have been proposed as possible sonosensitizers for sonodynamic therapy of cancer. Therefore, we evaluated the pharmacokinetic properties and sonosensitizing activities of 5-ALA and SnCe6 in vivo by using the EMT6/KU tumor-bearing chick embryos.

RESULTS:

The concentration of PpIX in tumor and liver tissues and serum increased in a time-dependent manner after the i.v. administration of 5-ALA; PpIX concentrations reached their peak level after 5-7 h. The concentration of SnCe6 reached its maximum value in the tumor tissue and serum immediately after i.v. administration. The combined treatment of 5-ALA or SnCe6 with ultrasound irradiation showed a significant antitumor effect towards EMT6/KU solid tumors.

CONCLUSION:

We evaluated the pharmacokinetic properties and sonosensitizing activities of 5-ALA and SnCe6 in a chick embryo model and found that 5-ALA might be more suitable as a sonosensitizer than SnCe6.

• Authors: Yoshihiro Uto, Dai Tamatani, Yusuke Mizuki, Yoshio Endo, Ikuo Nakanishi, Kei Ohkubo, Shunichi Fukuzumi, Masahiro Ishizuka, Tohru Tanaka, Daisuke Kuchiike, Kentaro Kubo, Toshio Inui, Hitoshi Hori
• Journal: Anticancer research (Anticancer Res) Vol. 34 Issue 8 Pg. 4583-7 (Aug 2014) ISSN: 1791-7530 [Electronic] Greece
• PMID: 25075103 (Publication Type: Journal Article)
• Copyright: Copyright© 2014 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

Chemical References

• Metalloporphyrins
• Protoporphyrins
• tin(IV) chlorin e6
• Aminolevulinic Acid
• protoporphyrin IX

Topics

• Aminolevulinic Acid (pharmacokinetics, pharmacology)
• Animals
• Cell Line, Tumor
• Chick Embryo
• Metalloporphyrins (pharmacokinetics, pharmacology)
• Mice
• Neoplasms, Experimental (therapy)
• Protoporphyrins (metabolism)
• Ultrasonic Therapy