Valproic acid attenuates the suppression of acetyl histone H3 and CREB activity in an inducible cell model of Machado-Joseph disease.

Abstract

Machado-Joseph disease (MJD) is caused by a (CAG)n trinucleotide repeat expansion that is translated into an abnormally long polyglutamine tract. This disease is considered the most common form of spinocerebellar ataxia (SCA). In the present study, we developed stable inducible cell lines (PC12Tet-On-Ataxin-3-Q28/84) expressing ataxin-3 with either normal or abnormal CAG repeats under doxycycline control. The expression of acetyl histone H3 and the induction of c-Fos in response to cAMP were strongly suppressed in cells expressing the protein with the expanded polyglutamine tract. Treatment with valproic acid, a histone deacetylase inhibitor (HDACi), attenuated mutant ataxin-3-induced cell toxicity and suppression of acetyl histone H3, phosphorylated cAMP-responsive element binding protein (p-CREB) as well as c-Fos expression. These results indicate that VPA can stimulate the up-regulation of gene transcription through hyperacetylation. Thus, VPA might have a therapeutic effect on MJD.

• Authors: X P Lin, L Feng, C G Xie, D B Chen, Z Pei, X L Liang, Q Y Xie, X H Li, S Y Pan
• Journal: International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience (Int J Dev Neurosci) Vol. 38 Pg. 17-22 (Nov 2014) ISSN: 1873-474X [Electronic] United States
• PMID: 25068645 (Publication Type: Journal Article)
• Copyright: Copyright © 2014 ISDN. Published by Elsevier Ltd. All rights reserved.

Chemical References

• Enzyme Inhibitors
• Histones
• Nerve Tissue Proteins
• Nuclear Proteins
• Peptides
• Proto-Oncogene Proteins c-fos
• Repressor Proteins
• Green Fluorescent Proteins
• polyglutamine
• Valproic Acid
• Nerve Growth Factor
• Cyclic AMP
• CREB-Binding Protein
• ATXN3 protein, human
• Ataxin-3

Topics

• Animals
• Ataxin-3
• CREB-Binding Protein (metabolism)
• Cell Differentiation (drug effects, physiology)
• Cell Proliferation (drug effects, genetics)
• Cyclic AMP (metabolism)
• Dose-Response Relationship, Drug
• Enzyme Inhibitors (pharmacology)
• Gene Expression Regulation (drug effects, genetics)
• Green Fluorescent Proteins (genetics, metabolism)
• Histones (metabolism)
• Humans
• Nerve Growth Factor (pharmacology)
• Nerve Tissue Proteins (genetics, metabolism)
• Nuclear Proteins (genetics, metabolism)
• PC12 Cells
• Peptides (metabolism)
• Proto-Oncogene Proteins c-fos (metabolism)
• Rats
• Repressor Proteins (genetics, metabolism)
• Transfection
• Trinucleotide Repeat Expansion (genetics)
• Valproic Acid (pharmacology)