Abstract |
The known functions of type II thioesterases (TEIIs) in type I polyketide synthases (PKSs) include selecting of starter acyl units, removal of aberrant extender acyl units, releasing of final products, and dehydration of polyketide intermediates. In this study, we characterized two TEIIs (ScnI and PKSIaTEII) from Streptomyces chattanoogensis L10. Deletion of scnI in S. chattanoogensis L10 decreased the natamycin production by about 43%. Both ScnI and PKSIaTEII could remove acyl units from the acyl carrier proteins (ACPs) involved in the natamycin biosynthesis. Our results show that the TEII could play important roles in both the initiation step and the elongation steps of a polyketide biosynthesis; the intracellular TEIIs involved in different biosynthetic pathways could complement each other.
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Authors | Yue-Yue Wang, Xin-Xin Ran, Wei-Bin Chen, Shui-Ping Liu, Xiao-Sheng Zhang, Yuan-Yang Guo, Xin-Hang Jiang, Hui Jiang, Yong-Quan Li |
Journal | FEBS letters
(FEBS Lett)
Vol. 588
Issue 17
Pg. 3259-64
(Aug 25 2014)
ISSN: 1873-3468 [Electronic] England |
PMID | 25064840
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
Chemical References |
- Natamycin
- Arginine
- Fatty Acid Synthases
- Thiolester Hydrolases
- thioesterase II
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Topics |
- Amino Acid Sequence
- Arginine
(metabolism)
- Fatty Acid Synthases
(chemistry, metabolism)
- Intracellular Space
(enzymology)
- Molecular Sequence Data
- Natamycin
(biosynthesis)
- Streptomyces
(cytology, enzymology, metabolism)
- Thiolester Hydrolases
(chemistry, metabolism)
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