MicroRNA expression profile of bromocriptine-resistant prolactinomas.
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| Abstract |
MicroRNAs (miRNA) have been implicated in the resistance of tumors to chemotherapy. However, little is known about miRNA expression in bromocriptine-resistant prolactinomas. In this study, 23 prolactinoma samples were classified as bromocriptine-sensitive or -resistant according to the clinical definition of bromocriptine resistance, and their miRNA expression profiles were determined using Solexa sequencing. We found 41 miRNAs that were differentially expressed between the two groups, and 12 of these were validated by stem-loop qRT-PCR. Hsa-mir-93, hsa-mir-17, hsa-mir-22*, hsa-mir-126*, hsa-mir-142-3p, hsa-mir-144*, hsa-mir-486-5p, hsa-mir-451, and hsa-mir-92a were up-regulated and hsa-mir-30a, hsa-mir-382, and hsa-mir-136 were down-regulated in bromocriptine-resistant prolactinomas in comparison with bromocriptine-sensitive prolactinomas. Furthermore, silencing of mir-93 significantly increased the sensitivity of MMQ cells to dopamine agonist treatment. Mir-93 directly affected p21 expression in MMQ cells by targeting the 3'-UTR. Our study is the first to identify a miRNA expression profile associated with bromocriptine-resistant prolactinoma.
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| Authors | Zhe Bao Wu, Wei Qiang Li, Shao Jian Lin, Cheng De Wang, Lin Cai, Jiang Long Lu, Yun Xiang Chen, Zhi Peng Su, Han Bing Shang, Wen Lei Yang, Wei Guo Zhao |
| Journal | Molecular and cellular endocrinology (Mol Cell Endocrinol) Vol. 395 Issue 1-2 Pg. 10-8 (Sep 2014) ISSN: 1872-8057 [Electronic] Ireland |
| PMID | 25064468 (Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | Copyright © 2014 Elsevier Ireland Ltd. All rights reserved. |
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