Brain tissue hypoxia and oxidative stress induced by obstructive apneas is different in young and aged rats.

Abstract

STUDY OBJECTIVES:

To test the hypotheses that brain oxygen partial pressure (PtO2) in response to obstructive apneas changes with age and that it might lead to different levels of cerebral tissue oxidative stress.

DESIGN:

Prospective controlled animal study.

SETTING:

University laboratory.

PARTICIPANTS:

Sixty-four male Wistar rats: 32 young (3 mo old) and 32 aged (18 mo).

INTERVENTIONS:

Protocol 1: Twenty-four animals were subjected to obstructive apneas (50 apneas/h, lasting 15 sec each) or to sham procedure for 50 min. Protocol 2: Forty rats were subjected to obstructive apneas or sham procedure for 4 h.

MEASUREMENTS AND RESULTS:

Protocol 1: Real-time PtO2 measurements were performed using a fast-response oxygen microelectrode. During successive apneas cerebral cortex PtO2 presented a different pattern in the two age groups; there was a fast increase in young rats, whereas it remained without significant changes between the beginning and the end of the protocol in the aged group. Protocol 2: Brain oxidative stress assessed by lipid peroxidation increased after apneas in young rats (1.34 ± 0.17 nmol/mg of protein) compared to old ones (0.63 ± 0.03 nmol/mg), where a higher expression of antioxidant enzymes was observed.

CONCLUSIONS:

The results suggest that brain oxidative stress in aged rats is lower than in young rats in response to recurrent apneas, mimicking obstructive sleep apnea. This could be due to the different PtO2 response observed between age groups and the increased antioxidant expression in aged rats.

CITATION:

Dalmases M, Torres M, Márquez-Kisinousky L, Almendros I, Planas AM, Embid C, Martínez-Garcia MA, Navajas D, Farré R, Montserrat JM. Brain tissue hypoxia and oxidative stress induced by obstructive apneas is different in young and aged rats.

• Authors: Mireia Dalmases, Marta Torres, Leonardo Márquez-Kisinousky, Isaac Almendros, Anna M Planas, Cristina Embid, Miguel Ángel Martínez-Garcia, Daniel Navajas, Ramon Farré, Josep Maria Montserrat
• Journal: Sleep (Sleep) Vol. 37 Issue 7 Pg. 1249-56 (Jul 01 2014) ISSN: 1550-9109 [Electronic] United States
• PMID: 25061253 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Biomarkers
• Glutathione
• Oxygen

Topics

• Aging (metabolism)
• Animals
• Antioxidants (metabolism)
• Biomarkers (metabolism)
• Brain (metabolism, physiopathology)
• Cerebral Cortex (metabolism, physiopathology)
• Glutathione (metabolism)
• Hypoxia, Brain (metabolism, physiopathology)
• Lipid Peroxidation
• Male
• Oxidative Stress
• Oxygen (metabolism)
• Partial Pressure
• Prospective Studies
• Rats
• Rats, Wistar
• Sleep Apnea, Obstructive (metabolism, physiopathology)