Regulatory T cells in the actinic cheilitis.

Actinic cheilitis (AC) is an oral potentially malignant lesion which is the counterpart of actinic keratosis of the skin and has potential to develop into squamous cell carcinoma. Regulatory T cells (Tregs) have a critical role in modulating the antitumor immune responses. The presence of regulatory T cells in potentially malignant lesions has not been described. We chose investigate the involvement of regulatory T cells in potentially malignant lesions.
The frequency, phenotype, and activity of CD4+CD25+ T cells isolated from blood and lesion of AC patients were analyzed by flow cytometry. Cytokines were quantified by ELISA. Data were compared with samples from healthy subjects.
The frequency and suppressor activity of circulating CD4+CD25+ T cells was similar in AC patients and control subjects. However, the frequencies of IL-10-positive Tregs were higher in AC patients, and these cells inhibited interferon-gamma (IFN-γ) and increased interleukin (IL)-10 productions in co-cultures. Furthermore, CD4+CD25+ T cells accumulate in AC lesions. Lesions-derived regulatory T cells suppressed lymphocyte proliferation and pro-inflammatory cytokine production. Moreover, high levels of IL-10 and transforming growth factor-β (TGF-β), and low IFN-γ were detected in the potentially malignant lesions.
Therefore, our data show that Tregs accumulate in AC lesions, and these cells could be suppressing immune responses in a potentially malignant microenvironment.
AuthorsThaís Helena Gasparoto, Tatiana Salles de Souza Malaspina, José Humberto Damante, Edgard Franco de Mello Jr, Maura Rosane Valério Ikoma, Gustavo Pompermaier Garlet, Maria Renata Sales Nogueira Costa, Karen Angélica Cavassani, João Santana da Silva, Ana Paula Campanelli
JournalJournal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology (J Oral Pathol Med) Vol. 43 Issue 10 Pg. 754-60 (Nov 2014) ISSN: 1600-0714 [Electronic] Denmark
PMID25060152 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
Chemical References
  • Antigens, CD4
  • IL10 protein, human
  • IL2RA protein, human
  • Inflammation Mediators
  • Interleukin-2 Receptor alpha Subunit
  • Transforming Growth Factor beta
  • Interleukin-10
  • Interferon-gamma
  • Adult
  • Aged
  • Aged, 80 and over
  • Antigens, CD4 (analysis)
  • CD4-Positive T-Lymphocytes (immunology)
  • Case-Control Studies
  • Cell Proliferation
  • Cheilitis (blood, immunology, pathology)
  • Humans
  • Inflammation Mediators (immunology)
  • Interferon-gamma (analysis)
  • Interleukin-10 (analysis)
  • Interleukin-2 Receptor alpha Subunit (analysis)
  • Leukocytes, Mononuclear (immunology)
  • Lip Neoplasms (immunology)
  • Lymphocyte Activation (immunology)
  • Lymphocyte Count
  • Middle Aged
  • Phenotype
  • Precancerous Conditions (immunology)
  • T-Lymphocytes, Regulatory (immunology, pathology)
  • Transforming Growth Factor beta (analysis)
  • Tumor Microenvironment (immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: