Abstract | PURPOSE: METHODS: Our study is a prospective case series of 20 consecutive patients with BRVO and 10 consecutive patients serving as controls. Vitreous taps were extracted from the central vitreous body and plasminogen was functionally determined in an innovative, ultrasensitive p-nitroanilide reaction after activation with streptokinase (100% of normal, %N = functional plasminogen in pooled normal citrated plasma). Intravitreal VEGF levels were assayed to estimate BRB breakdown. RESULTS: Intravitreal functional plasminogen was detected in all analyzed samples ( n = 30) and mean (±SD) plasminogen activities were found to be 0.97 ± 1.06%N (range: 0.03-3.9%N). Patients suffering from BRVO exhibited significantly higher intravitreal plasminogen (1.35 ± 1.11%N) in comparison with controls (0.20 ± 0.21%N, p < 0.001). Intravitreal VEGF concentrations in the BRVO group (576 ± 547 pg/ml) were significantly higher than these in controls (111 ± 120 pg/ml, p = 0.003). There was a significant correlation between intravitreal functional plasminogen and intravitreal VEGF levels (r = 0.519, p = 0.003). CONCLUSIONS:
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Authors | Thomas Bertelmann, Walter Sekundo, Stefan Strodthoff, Michael C P Witteborn, Thomas Stief, Sebastian Irle, Nauke Nguyen, Michael J Koss, Stefan Mennel |
Journal | Ophthalmic research
(Ophthalmic Res)
Vol. 52
Issue 2
Pg. 74-80
( 2014)
ISSN: 1423-0259 [Electronic] Switzerland |
PMID | 25059575
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 S. Karger AG, Basel. |
Chemical References |
- Fibrinolytic Agents
- VEGFA protein, human
- Vascular Endothelial Growth Factor A
- Plasminogen
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Topics |
- Aged
- Aged, 80 and over
- Blood-Retinal Barrier
(physiology)
- Capillary Permeability
- Enzyme-Linked Immunosorbent Assay
- Female
- Fibrinolytic Agents
(metabolism)
- Humans
- Male
- Middle Aged
- Plasminogen
(metabolism)
- Prospective Studies
- Retinal Vein Occlusion
(metabolism)
- Vascular Endothelial Growth Factor A
(metabolism)
- Vitreous Body
(metabolism)
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