miR-101 promotes breast cancer cell apoptosis by targeting Janus kinase 2.

Abstract

AIMS:

microRNA-101 (miR-101) is down-regulated in several cancers. In this study, we explored the effects of dysregulated miR-101 on breast cancer cells and the underlying mechanisms.

METHODS:

miR-101 level was quantified by real-time RT-PCR. Cell viability was analyzed by MTT assay. Apoptosis was detected by flow cytometry and TUNEL assay. Moreover, the level of protein expression was determined by Western blot.

RESULTS:

miR-101 level was markedly reduced in both the human breast cancer samples and cultured breast cancer cell lines (MCF-7, MDA-MB-231). Overexpression of miR-101 inhibited the proliferation and promoted the apoptosis in cultured MCF-7 and MDA-MB-231 cells, which were reversed by co-transfection of AMO-101, the inhibitor of miR-101. We validated Janus kinase 2 (Jak2) as a direct target of miR-101. Knockdown of Jak2 induced apoptosis in cultured breast cancer cells. Moreover, the level of miR-101 is negatively correlated with Jak2 in breast cancer tissues and cell lines.

CONCLUSIONS:

miR-101 suppressed proliferation and promoted apoptosis in breast cancer cells by targeting Jak2. These findings indicate that manipulation of miR-101 expression may represent a novel therapeutic strategy in the treatment of breast cancer.

• Authors: Lu Wang, Linqiang Li, Rui Guo, Xuelian Li, Ying Lu, Xiaoxiang Guan, Samuel Chege Gitau, Leimin Wang, Chaoqian Xu, Baofeng Yang, Hongli Shan
• Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (Cell Physiol Biochem) Vol. 34 Issue 2 Pg. 413-22 ( 2014) ISSN: 1421-9778 [Electronic] Germany
• PMID: 25059472 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 S. Karger AG, Basel.

Chemical References

• MIRN101 microRNA, human
• MicroRNAs
• Janus Kinase 2

Topics

• Apoptosis (genetics, physiology)
• Blotting, Western
• Breast Neoplasms (enzymology, pathology)
• Cell Line, Tumor
• Female
• Flow Cytometry
• Humans
• In Situ Nick-End Labeling
• Janus Kinase 2 (genetics)
• MicroRNAs (physiology)
• Real-Time Polymerase Chain Reaction