Methazolamide-loaded solid lipid nanoparticles modified with low-molecular weight chitosan for the treatment of glaucoma: vitro and vivo study.

Abstract

The aims of this study were to design and characterize methazolamide (MTZ)-loaded solid lipid nanoparticles (SLN) with and without modification of low molecular weight chitosan (CS) and compare their potentials for ocular drug delivery. Low molecular weight CS was obtained via a modified chemical oxidative degradation method. SLN with CS (CS-SLN-MTZ) and without CS (SLN-MTZ) were prepared according to a modified emulsion-solvent evaporation method. SLN-MTZ and CS-SLN-MTZ were 199.4 ± 2.8 nm and 252.8 ± 4.0 nm in particle size, -21.3 ± 1.9 mV and +31.3 ± 1.7 mV in zeta potential, respectively. Physical stability studies demonstrated that CS-SLN-MTZ remained stable for at least 4 months at 4 °C, while SLN-MTZ no more than 2 months. A prolonged in vitro release profile of MTZ from CS-SLN-MTZ was obtained compared with SLN-MTZ. Furthermore, CS-SLN-MTZ presented a better permeation property in excised rabbit cornea. In vivo studies indicated that the intraocular pressure lowering effect of CS-SLN-MTZ (245.75 ± 18.31 mmHg × h) was significantly better than both SLN-MTZ (126.74 ± 17.73 mmHg × h) and commercial product Brinzolamide Eye Drops AZOPT® (171.17 ± 16.45 mmHg × h). The maximum percentage decrease in IOP of CS-SLN-MTZ (42.78 ± 7.71%) was higher than SLN-MTZ (27.82 ± 4.15%) and was comparable to AZOPT (38.06 ± 1.25%). CS-SLN-MTZ showed no sign of ocular irritancy according to the Draize method and the histological examination.

• Authors: Fengzhen Wang, Li Chen, Dongsheng Zhang, Sunmin Jiang, Kun Shi, Yuan Huang, Rui Li, Qunwei Xu
• Journal: Journal of drug targeting (J Drug Target) Vol. 22 Issue 9 Pg. 849-58 (Nov 2014) ISSN: 1029-2330 [Electronic] England
• PMID: 25045926 (Publication Type: Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)

Chemical References

• Drug Carriers
• Lipids
• Chitosan
• Methazolamide

Topics

• Animals
• Chitosan (chemistry)
• Cornea (metabolism)
• Double-Blind Method
• Drug Carriers
• Drug Stability
• Female
• Glaucoma (drug therapy)
• Hydrogen-Ion Concentration
• Lipids (chemistry)
• Male
• Methazolamide (administration & dosage, pharmacology)
• Molecular Weight
• Nanoparticles (chemistry)
• Particle Size
• Rabbits