Theranostic nanoparticles based on superparamagnetic
iron oxide (
SPIO) have a great promise for
tumor diagnosis and gene therapy. However, the availability of
theranostic nanoparticles with efficient gene transfection and minimal toxicity remains a big challenge. In this study, we construct an intelligent
SPIO-based nanoparticle comprising a
SPIO inner core and a
disulfide-containing
polyethylenimine (SSPEI) outer layer, which is referred to as a SSPEI-
SPIO nanoparticle, for redox-triggered gene release in response to an intracellular reducing environment. We reveal that SSPEI-
SPIO nanoparticles are capable of binding genes to form nano-complexes and mediating a facilitated gene release in the presence of
dithiothreitol (5-20 mM), thereby leading to high transfection efficiency against different
cancer cells. The SSPEI-
SPIO nanoparticles are also able to deliver
small interfering RNA (
siRNA) for the silencing of human
telomerase reverse transcriptase genes in HepG2 cells, causing their apoptosis and growth inhibition. Further, the nanoparticles are applicable as T2-negative
contrast agents for magnetic resonance (MR) imaging of a
tumor xenografted in a nude mouse. Importantly, SSPEI-
SPIO nanoparticles have relatively low cytotoxicity in vitro at a high concentration of 100 μg/mL. The results of this study demonstrate the utility of a
disulfide-containing cationic
polymer-decorated
SPIO nanoparticle as highly potent and low-toxic
theranostic nano-system for specific
nucleic acid delivery inside
cancer cells.