Lack of effect of a P2Y6 receptor antagonist on neuropathic pain behavior in mice.

Abstract

Accumulating evidence indicates that various subtypes of purinergic receptors (P2X and P2Y receptor families) play an essential role in the development and the maintenance of neuropathic pain. However, there is only limited data available about the role of P2Y6 receptors in pain processing. Here we detected P2Y6 receptor immunoreactivity in primary afferent neurons of mice and observed an upregulation in response to peripheral nerve injury. However, systemic and intrathecal administration of the P2Y6 receptor antagonist MRS2578 failed to affect the injury-induced neuropathic pain behavior. Our results suggest that P2Y6 receptors, in contrast to other purinergic receptor subtypes, are not critically involved in nerve injury-induced neuropathic pain processing in mice.

• Authors: Katharina Martina Janice Syhr, Wiebke Kallenborn-Gerhardt, Ruirui Lu, Katrin Olbrich, Katja Schmitz, Julia Männich, Nerea Ferreiros-Bouzas, Gerd Geisslinger, Ellen Niederberger, Achim Schmidtko
• Journal: Pharmacology, biochemistry, and behavior (Pharmacol Biochem Behav) Vol. 124 Pg. 389-95 (Sep 2014) ISSN: 1873-5177 [Electronic] United States
• PMID: 25042778 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• Isothiocyanates
• N,N''-1,4-butanediylbis(N'-(3-isothiocyanatophenyl))thiourea
• Purinergic Antagonists
• Receptors, Purinergic P2
• purinoceptor P2Y6
• Thiourea

Topics

• Animals
• Behavior, Animal
• Blotting, Western
• Isothiocyanates (therapeutic use)
• Male
• Mice
• Mice, Inbred C57BL
• Neuralgia (drug therapy)
• Purinergic Antagonists (therapeutic use)
• Real-Time Polymerase Chain Reaction
• Receptors, Purinergic P2 (drug effects, metabolism)
• Spinal Cord (metabolism)
• Thiourea (analogs & derivatives, therapeutic use)