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Superoxide dismutase enzymosomes: carrier capacity optimization, in vivo behaviour and therapeutic activity.

AbstractPURPOSE:
A strategy not usually used to improve carrier-mediated delivery of therapeutic enzymes is the attachment of the enzymes to the outer surface of liposomes. The aim of our work was to design a new type of enzymosomes with a sufficient surface-exposed enzyme load while preserving the structural integrity of the liposomal particles and activity of the enzyme.
METHODS:
The therapeutic antioxidant enzyme superoxide dismutase (SOD) was covalently attached to the distal terminus of polyethylene glycol (PEG) polymer chains, located at the surface of lipid vesicles, to obtain SOD-enzymosomes.
RESULTS:
The in vivo fate of the optimized SOD-enzymosomes showed that SOD attachment at the end of the activated PEG slightly reduced the residence time of the liposome particles in the bloodstream after IV administration. The biodistribution studies showed that SOD-enzymosomes had a similar organ distribution profile to liposomes with SOD encapsulated in their aqueous interior (SOD-liposomes). SOD-enzymosomes showed earlier therapeutic activity than both SOD-liposomes and free SOD in rat adjuvant arthritis. SOD-enzymosomes, unlike SOD-liposomes, have a therapeutic effect, decreasing liver damage in a rat liver ischemia/reperfusion model.
CONCLUSIONS:
SOD-enzymosomes were shown to be a new and successful therapeutic approach to oxidative stress-associated inflammatory situations/diseases.
AuthorsM Luísa Corvo, H Susana Marinho, Paulo Marcelino, Rui M Lopes, Carlos A Vale, Claúdia R Marques, Luísa C D Martins, Peter Laverman, Gert Storm, M Bárbara A F Martins
JournalPharmaceutical research (Pharm Res) Vol. 32 Issue 1 Pg. 91-102 (Jan 2015) ISSN: 1573-904X [Electronic] United States
PMID25037861 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Drug Carriers
  • Liposomes
  • Polyethylene Glycols
  • Superoxide Dismutase
Topics
  • Animals
  • Arthritis, Experimental (drug therapy, metabolism)
  • Drug Carriers (chemistry)
  • Drug Compounding
  • Drug Liberation
  • Liposomes
  • Liver (blood supply)
  • Male
  • Oxidative Stress (drug effects)
  • Particle Size
  • Polyethylene Glycols (chemistry)
  • Rats, Wistar
  • Reperfusion Injury (drug therapy, metabolism)
  • Superoxide Dismutase (administration & dosage, pharmacokinetics, therapeutic use)
  • Surface Properties
  • Tissue Distribution
  • Treatment Outcome

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