Determination by UPLC-MS of four dammarane-type saponins from heat-processed Gynostemma pentaphyllum.

Abstract	Heat-processed Gynostemma pentaphyllum and its main dammaran-type saponins, gypenoside L, gypenoside LI, damulin B, and damulin A, possess non-small cell lung carcinoma A549 cell inhibitory activity. We established in this study a method by ultra-high performance liquid chromatography with tandem mass spectrometry for determination of the saponins and also investigated their content change in heat-processed G. pentaphyllum. The main saponins increased with increasing heating temperature and time. Further investigation showed that they were produced from gypenoside XLVI and gypenoside LVI by undergoing hydrolysis during the heat treatment.
Authors	Qian Wu, Moonhee Jang, Xiang-Lan Piao
Journal	Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 78 Issue 2 Pg. 311-6 ( 2014) ISSN: 1347-6947 [Electronic] England
PMID	25036687 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Saponins Triterpenes dammarane
Topics	Cell Line, Tumor Chromatography, High Pressure Liquid Gynostemma (metabolism) Hot Temperature Humans Hydrolysis Saponins (chemistry, metabolism) Triterpenes (chemistry)

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