Oxymatrine lightened the inflammatory response of LPS-induced mastitis in mice through affecting NF-κB and MAPKs signaling pathways.

Abstract

Mastitis, an inflammatory reaction of the mammary gland, is recognized as one of the most costly diseases in dairy cattle. Oxymatrine, one of the alkaloids extracted from Chinese herb Sophora flavescens Ait, has been reported to have many biological activities, such as anti-inflammatory, anti-virus, and anti-hepatic fibrosis properties. The aim of this study was to investigate the protective effect and the anti-inflammatory mechanism of oxymatrine on lipopolysaccharide (LPS)-induced mastitis in mice. The mouse mastitis was induced by 10 μg of LPS for 24 h. Oxymatrine was intraperitoneally administered with the dose of 30, 60, and 120 mg/kg 1 h before and 12 h after LPS induction. The results showed that oxymatrine significantly attenuated the damage of the mammary gland induced by LPS. Oxymatrine inhibited the phosphorylation of NF-κB p65 and IκB in NF-κB signal pathway and reduced the phosphorylation of p38, ERK, and JNK in mitogen-activated protein kinase (MAPKs) signal pathway. The results showed that oxymatrine had a protective effect on LPS-induced mastitis, and the anti-inflammatory mechanism of oxymatrine was related to the inhibition of NF-κB and MAPKs signal pathways.

• Authors: Zhengtao Yang, Ronglan Yin, Yunfeng Cong, Zhanqing Yang, Ershun Zhou, Zhengkai Wei, Zhicheng Liu, Yongguo Cao, Naisheng Zhang
• Journal: Inflammation (Inflammation) Vol. 37 Issue 6 Pg. 2047-55 (Dec 2014) ISSN: 1573-2576 [Electronic] United States
• PMID: 25034832 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Alkaloids
• Anti-Inflammatory Agents
• Inflammation Mediators
• Lipopolysaccharides
• NF-kappa B
• Quinolizines
• oxymatrine

Topics

• Alkaloids (pharmacology, therapeutic use)
• Animals
• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Dose-Response Relationship, Drug
• Female
• Inflammation (drug therapy, metabolism)
• Inflammation Mediators (antagonists & inhibitors, metabolism)
• Lipopolysaccharides (toxicity)
• MAP Kinase Signaling System (drug effects, physiology)
• Male
• Mastitis (drug therapy, metabolism, pathology)
• Mice
• Mice, Inbred BALB C
• NF-kappa B (antagonists & inhibitors, metabolism)
• Quinolizines (pharmacology, therapeutic use)
• Signal Transduction (drug effects, physiology)
• Sophora