Protective effect of veratric acid on lipopolysaccharide-induced acute lung injury in mice.

Abstract

Veratric acid, a simple benzoic acid derived from plants and fruits, has been reported to exhibit antibacterial, anti-inflammatory and other important therapeutic activities. The aim of this study was to detect the effects of veratric acid on LPS-induced acute lung injury and to investigate the effects of veratric acid on NF-κB signaling pathway. Male BALB/c mice were pretreated with dexamethasone or veratric acid 1h before intranasal instillation of LPS. 7h after LPS administration, the myeloperoxidase in lung tissues, lung wet/dry weight ratio and inflammatory cells in the bronchoalveolar lavage fluid (BALF) were determined. The effects of veratric acid on pro-inflammatory cytokines and signal pathways were analyzed by enzyme-linked immunosorbent assay (ELISA) and Western blotting. The results showed that veratric acid inhibited LPS-induced TNF-α, IL-6 and IL-1β production in a dose dependent manner. It was also observed that veratric acid attenuated lung histopathologic changes. The wet/dry weight ratio of lungs and the number of total cells, neutrophils, macrophages in the BALF were all decreased. Furthermore, veratric acid inhibited the phosphorylation of NF-κB p65 and IκB. These results indicate that veratric acid inhibits NF-κB signaling pathways to attenuate inflammatory injury induced by LPS. Veratric acid may be a potential therapeutic reagent for acute lung injury treatment.

• Authors: Xu Ran, Shang Chao, Zhao Jun-Gang, Han Yun, Chen Kuan-Bing, Shi Wen-Jun
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 740 Pg. 227-32 (Oct 05 2014) ISSN: 1879-0712 [Electronic] Netherlands
• PMID: 25034806 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Interleukin-1beta
• Interleukin-6
• Lipopolysaccharides
• Tumor Necrosis Factor-alpha
• veratric acid
• Peroxidase
• Vanillic Acid

Topics

• Acute Lung Injury (chemically induced, drug therapy, immunology, pathology)
• Animals
• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Bronchoalveolar Lavage Fluid (cytology, immunology)
• Interleukin-1beta (immunology)
• Interleukin-6 (immunology)
• Lipopolysaccharides
• Lung (drug effects, immunology, pathology)
• Male
• Mice, Inbred BALB C
• Organ Size (drug effects)
• Peroxidase (immunology)
• Tumor Necrosis Factor-alpha (immunology)
• Vanillic Acid (analogs & derivatives, pharmacology, therapeutic use)