Interaction of PTPRO and TLR4 signaling in hepatocellular carcinoma.

Abstract

Protein tyrosine phosphatase receptor type O (PTPRO) has been identified as a tumor suppressor in a number of cancers including hepatocellular carcinoma (HCC). Toll-like receptor 4 (TLR4) plays diverse roles in HCC tumorigenesis and progression. The association between PTPRO and TLR4 signaling in HCC remains largely unknown. We aimed to clarify the interaction between PTPRO and TLR4 in HCC. Surprisingly, we found reduced and positive-related expression of TLR4 and PTPRO in 84 human HCC specimens. Increased TLR4 expression and activity was found in PTPRO-overexpressed HCC cells stimulated with lipopolysaccharide (LPS). The feedback regulation of PTPRO and TLR4 was dependent on nuclear factor-κB (NF-κB) activation, as suggested by NF-κB inhibition and luciferase reporter assay. Our study suggests that the effect of PTPRO on TLR4 signaling is dependent on NF-κB pathway, suggesting an interesting PTPRO/TLR4/NF-κB signaling feedback loop in HCC carcinogenesis and progression.

• Authors: Donghua Xu, Xiaochen Wang, Shushan Yan, Yin Yin, Jiajie Hou, Xuehao Wang, Beicheng Sun
• Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (Tumour Biol) Vol. 35 Issue 10 Pg. 10267-73 (Oct 2014) ISSN: 1423-0380 [Electronic] Netherlands
• PMID: 25034527 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• NF-kappa B
• TLR4 protein, human
• Toll-Like Receptor 4
• PTPRO protein, human
• Receptor-Like Protein Tyrosine Phosphatases, Class 3

Topics

• Blotting, Western
• Carcinoma, Hepatocellular (metabolism)
• Cell Line, Tumor
• Fluorescent Antibody Technique
• Humans
• Liver Neoplasms (metabolism)
• NF-kappa B (metabolism)
• Real-Time Polymerase Chain Reaction
• Receptor-Like Protein Tyrosine Phosphatases, Class 3 (metabolism)
• Signal Transduction (physiology)
• Toll-Like Receptor 4 (metabolism)
• Transfection