Altered CD8(+) T cell frequency and function in tuberculous lymphadenitis.

Abstract

CD8(+) T cells secreting Type1 and Type 17 cytokines and cytotoxic molecules play a major role in immunity and protection against pulmonary tuberculosis (PTB), although their role in tuberculous lymphadenitis (TBL) is not well known. To identify the distribution and function of CD8(+) T cells expressing Type1, Type 2 and Type 17 cytokines and cytotoxic molecules in TBL, we examined baseline and mycobacterial-antigen specific immune responses in the whole blood of individuals with PTB and compared them with TBL. TBL is characterized by elevated frequencies of baseline and mycobacterial-antigen stimulated CD8(+) T cells expressing Type 1 (IL-2 and TNFα) and Type 17 (IL-17A and IL-17F) cytokines in comparison to PTB individuals. In contrast, TBL individuals exhibited diminished frequency of CD8(+) T cells expressing perforin, granzyme B and CD107a. The blockade of IL-1R and IL-6R during antigenic stimulation resulted in significantly diminished frequencies of CD8(+) T cells expressing Type 1 and Type 17 cytokines in TBL. Therefore, our data suggest that TBL is characterized by an IL-1 and IL-6 dependent expansion of CD8(+) T cells expressing Type 1 and Type 17 cytokines as well as altered frequencies of cytotoxic molecules, reflecting an important association of these cells with the pathogenesis of TBL.

• Authors: Nathella Pavan Kumar, Rathinam Sridhar, Luke Elizabeth Hanna, Vaithilingam V Banurekha, Mohideen S Jawahar, Thomas B Nutman, Subash Babu
• Journal: Tuberculosis (Edinburgh, Scotland) (Tuberculosis (Edinb)) Vol. 94 Issue 5 Pg. 482-93 (Sep 2014) ISSN: 1873-281X [Electronic] Scotland
• PMID: 25027793 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ltd. All rights reserved.

Chemical References

• Biomarkers
• Cytokines

Topics

• Adolescent
• Adult
• Biomarkers (blood)
• CD8-Positive T-Lymphocytes (immunology, metabolism, microbiology)
• Cells, Cultured
• Cytokines (blood)
• Female
• Host-Pathogen Interactions
• Humans
• Immunologic Memory
• Immunophenotyping
• Lymphocyte Activation
• Lymphocyte Count
• Male
• Middle Aged
• Tuberculosis, Lymph Node (blood, diagnosis, immunology, microbiology)
• Tuberculosis, Pulmonary (blood, diagnosis, immunology, microbiology)
• Young Adult