MicroRNA-1290 promotes asiatic acid‑induced apoptosis by decreasing BCL2 protein level in A549 non‑small cell lung carcinoma cells.

Abstract

Asiatic acid, a triterpenoid derived from Centella asiatica, is a putative anticancer agent in several types of cancer cells. Investigations of its biological role in negative regulation of cell growth have focused on the extent of induction of apoptosis in a cell-type-specific manner. In this study, we identified an important regulator of asiatic acid-induced cell death, microRNA (miR)-1290, which sensitizes cells to asiatic acid-induced cytotoxicity and negatively regulates BCL2 expression. Asiatic acid significantly upregulated miR-1290, and asiatic acid-induced cell death was shown to be dependent on miR-1290 activity. Molecular assays demonstrated that BCL2 mRNA is a direct target of miR-1290-mediated RNA interference. The results of functional studies suggest that miR-1290 suppresses cell viability and cell cycle progression. These data provide insight into miR-1290-mediated cellular mechanisms in asiatic acid-treated A549 non-small cell lung carcinoma cells.

• Authors: Ki Bbeum Kim, Karam Kim, Seunghee Bae, Yeonghmin Choi, Hwa Jun Cha, Soo Yeon Kim, Jae Ho Lee, So Hyeon Jeon, Ho Jung Jung, Kyu Joong Ahn, In-Sook An, Sungkwan An
• Journal: Oncology reports (Oncol Rep) Vol. 32 Issue 3 Pg. 1029-36 (Sep 2014) ISSN: 1791-2431 [Electronic] Greece
• PMID: 25016979 (Publication Type: Journal Article)

Chemical References

• 3' Untranslated Regions
• Antineoplastic Agents
• BCL2 protein, human
• MIRN1290 microRNA, human
• MicroRNAs
• Pentacyclic Triterpenes
• Proto-Oncogene Proteins c-bcl-2
• asiatic acid

Topics

• 3' Untranslated Regions
• Antineoplastic Agents (pharmacology)
• Apoptosis
• Carcinoma, Non-Small-Cell Lung (genetics, pathology)
• Cell Cycle (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Humans
• Lung Neoplasms (genetics, pathology)
• MicroRNAs (genetics)
• Pentacyclic Triterpenes (pharmacology)
• Proto-Oncogene Proteins c-bcl-2 (genetics)
• Up-Regulation